Exercise 12: Serological Testing: Activity 2: Comparing Samples with Ouchterlony Double Diffusion Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. When two antigens are identical their precipitin lines form You correctly answered: c. an arc 2. Antigen and antibody move toward each other because of You correctly answered: b. diffusion 3. If two antigens form a spur, they You correctly answered: d. have partial identity 4. The Ouchterlony test relies on the formation of a(n) You correctly answered: a. precipitate
Experiment Results Predict Question: Predict Question: How do you think human serum albumin and bovine serum albumin will compare? Your answer : b. They will have partial identity Stop & Think Questions: Which of the following best describes the two solutions you just added to well 1? You correctly answered: c. They are both antibodies produced by a goat. Experiment Data: Wells 2 and 5 2 and 3 3 and 4 4 and 5 Identity Identity Partial Non-Identity Non-Identity
Post-lab Quiz Results You scored 50% by answering 2 out of 4 questions correctly. 1. There is a spur between wells 2 and 5 and between wells 3 and 4 because the You correctly answered: d. antigens have partial identity. 2. What is the unknown antigen in well 5 (recall that well 2 contains bovine serum albumin and well 4 contains human serum albumin)? Your answer: d. goat antibodies Correct answer: b. human serum albumin 3. Which of the following best describes this test? You correctly answered: c. Antibodies are used to detect the presence of antigens. 4. The antigen-antibody precipitate forms where the ratio of antigen and antibody is Your answer: c. even. Correct answer: d. optimum.
Review Sheet Results 1. Describe how you were able to determine what antigen is in the unknown well. You did not answer this question. 2. Why does the precipitin line form? You did not answer this...
Please join StudyMode to read the full document