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Physio Ex 9.0 Exercise 12 Activity 1

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Physio Ex 9.0 Exercise 12 Activity 1
Exercise 12: Serological Testing: Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. Which of the following has an epitope?
You correctly answered: b. an antigen
2. Chlamydia trachomatis is
You correctly answered: c. a bacterium that reproduces inside its host cell.
3. The infectious form of Chlamydia that is spread from person to person is the
You correctly answered: d. elementary body that stains green in this activity
4. The goal of the direct fluorescent antibody test is to test for the presence of
You correctly answered: c. antigen.

10/15/13

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Experiment Results
Stop & Think Questions:
The source of the patient samples is
You correctly answered: c. epithelial scrapings
What is the name for the specific part of the antigen that the antibody binds to?
You correctly answered: b. epitope
15. Drag the first slide (patient A) to the fluorescent microscope.
Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies in the field below, and then click Submit Data to display your results in the grid. After you click Submit Data, the slide will automatically be placed in the biohazardous waste disposal.
You answered: 0 elementary bodies
16. Drag the next slide (patient B) to the fluorescent microscope.
Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies in the field below, and then click Submit Data to display your results in the grid. After you click Submit Data, the slide will automatically be placed in the biohazardous waste disposal.
You answered: 15 elementary bodies
17. Drag the next slide (patient C) to the fluorescent microscope.
Count the number of elementary bodies you see through the microscope (recall that

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