Photosynthesis & Cellular Respiration Lab
PHOTOSYNTHESIS
&
CELLUAR RESPIRATION LAB REPORT
% Absorption 400 425 450 475 500 525 550 575 600 625 650 675 700
Average 47 44 37 27 14 10 8 9 9 10 15 17 14
Use the graph of Average % Absorption for Grass Pigments to answer the following questions. ANSWER ONLY THE QUESTIONS BELOW. YOU WILL LOSE POINTS IF YOUR ANSWER INCLUDES UNRELATED INFORMATION.
1. What specific range of wavelengths explains why grass is green?
The absorption of all wavelengths outside approximately the 510 nm to just over the 625 nm range and the reflection within that wavelength range produces the effect the eyes see the brain interprets that grass is green.
2. Explain what specific characteristic of this range of wavelengths explains why grass is green?
Grass is green because of absorption from light energy “photons” carried out by chlorophyll, a green pigment. Chlorophylls are indisputably the dominant pigment masking the reflective wavelength properties of yellow, orange, and red pigments. Chlorophylls absorb all the visible light wavelengths below 510nm and a fraction of the visible light wavelengths above 625nm. Explaining why grass appears green, the chlorophyll is green because it reflects the green wavelengths the most.
3. NADI ASSAY RESULTS – You may adjust the table columns widths to your
Tube
Initial color & final color
Format: (initial/final) Designate each tube as a “CONTROL” or “EXPERIMENTAL”
For ‘CONTROL’ tubes – explain the purpose for control
For ‘EXPERIMENTAL’ tubes – state the purpose, results AND interpret the results of the experiment. Control or Experiment Purpose (Controls)
Purpose and Results (Experimental)
1 Milkish white/ milkish white Control To control for color change of the mitochondrial enzymes alone without addition of NADI. (false +)
2 Milkish white/ Bright pink to bluish purple Experiment Assay for Cytochrome oxidase activity. (+ purple) Shows the effects from the addition of NADI, a very
&
CELLUAR RESPIRATION LAB REPORT
% Absorption 400 425 450 475 500 525 550 575 600 625 650 675 700
Average 47 44 37 27 14 10 8 9 9 10 15 17 14
Use the graph of Average % Absorption for Grass Pigments to answer the following questions. ANSWER ONLY THE QUESTIONS BELOW. YOU WILL LOSE POINTS IF YOUR ANSWER INCLUDES UNRELATED INFORMATION.
1. What specific range of wavelengths explains why grass is green?
The absorption of all wavelengths outside approximately the 510 nm to just over the 625 nm range and the reflection within that wavelength range produces the effect the eyes see the brain interprets that grass is green.
2. Explain what specific characteristic of this range of wavelengths explains why grass is green?
Grass is green because of absorption from light energy “photons” carried out by chlorophyll, a green pigment. Chlorophylls are indisputably the dominant pigment masking the reflective wavelength properties of yellow, orange, and red pigments. Chlorophylls absorb all the visible light wavelengths below 510nm and a fraction of the visible light wavelengths above 625nm. Explaining why grass appears green, the chlorophyll is green because it reflects the green wavelengths the most.
3. NADI ASSAY RESULTS – You may adjust the table columns widths to your
Tube
Initial color & final color
Format: (initial/final) Designate each tube as a “CONTROL” or “EXPERIMENTAL”
For ‘CONTROL’ tubes – explain the purpose for control
For ‘EXPERIMENTAL’ tubes – state the purpose, results AND interpret the results of the experiment. Control or Experiment Purpose (Controls)
Purpose and Results (Experimental)
1 Milkish white/ milkish white Control To control for color change of the mitochondrial enzymes alone without addition of NADI. (false +)
2 Milkish white/ Bright pink to bluish purple Experiment Assay for Cytochrome oxidase activity. (+ purple) Shows the effects from the addition of NADI, a very