Mico Analysis
Microbiology
Abstract: The purpose of this lab was to use the Kirby-Bauer method to test the sensitivity of Staphylococcus epidermis of specific areas on a petri dish. The antibiotics were gentamicin, novobiacin, and penicillin. This resembled a culture and sensitivity test that is preformed at the hospital’s laboratory to find out the resistance of a microorganism. This experiment was meant to use the naked eye and a magnifying glass when necessary.
Procedures: This experiment called for prepared agar in a petri dish, smeared s. epidermis on the surface of the prepared agar, and a 3 separate quadrants labeled gentamicin, novobiacin, and penicillin. Once those steps were completed a tablet of the 3 antibiotics were placed in the center of the 3 quadrants with the petri dish lid closed. We then placed the petri dish in the incubator for 24-48 hours at 37 degree for growth.
Exercise 1: Antibiotic Sensitivity
Observations: The antibiotics all had a measurement above 5mm with one susceptible antibiotic, resulting in 2 resistant antibiotics.
Quadrant #1: Gentamicin
Susceptible (sensitive) with a measurement of 22mm
Quadrant #2: Novobiacin
Resistant with a measurement of 14mm
Quadrant #3: Penicillin
Resistant with a measurement of 7mm
Errors: This lab had few errors, and our results were very accurate to what we should have experienced. The error that was most frustrating was an error with our camera causing us not to be able to take pictures of our results.
Conclusions: This lab was very interesting and very cool to preform because we talk about bacteria and its resistance all the time, but never get to actually see it. It was also interesting to understand the concept of bacteria becoming resistant and becoming part of that potential “super bug” category. Overall this lab was both educational and fun to observe.
Lab Pictures for #10
Abstract: The purpose of this lab was to use the Kirby-Bauer method to test the sensitivity of Staphylococcus epidermis of specific areas on a petri dish. The antibiotics were gentamicin, novobiacin, and penicillin. This resembled a culture and sensitivity test that is preformed at the hospital’s laboratory to find out the resistance of a microorganism. This experiment was meant to use the naked eye and a magnifying glass when necessary.
Procedures: This experiment called for prepared agar in a petri dish, smeared s. epidermis on the surface of the prepared agar, and a 3 separate quadrants labeled gentamicin, novobiacin, and penicillin. Once those steps were completed a tablet of the 3 antibiotics were placed in the center of the 3 quadrants with the petri dish lid closed. We then placed the petri dish in the incubator for 24-48 hours at 37 degree for growth.
Exercise 1: Antibiotic Sensitivity
Observations: The antibiotics all had a measurement above 5mm with one susceptible antibiotic, resulting in 2 resistant antibiotics.
Quadrant #1: Gentamicin
Susceptible (sensitive) with a measurement of 22mm
Quadrant #2: Novobiacin
Resistant with a measurement of 14mm
Quadrant #3: Penicillin
Resistant with a measurement of 7mm
Errors: This lab had few errors, and our results were very accurate to what we should have experienced. The error that was most frustrating was an error with our camera causing us not to be able to take pictures of our results.
Conclusions: This lab was very interesting and very cool to preform because we talk about bacteria and its resistance all the time, but never get to actually see it. It was also interesting to understand the concept of bacteria becoming resistant and becoming part of that potential “super bug” category. Overall this lab was both educational and fun to observe.
Lab Pictures for #10