Preview

lab report on enzymes

Good Essays
Open Document
Open Document
570 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
lab report on enzymes
Enzymes

What Are Enzymes?

Substances that speed up chemical reactions are called catalysts. Organic catalysts are called enzymes.

Enzymes are specific for one particular reaction or group of related reactions.

Many reactions cannot occur without the correct enzyme present.

They are often named by adding "ase" to the name of the substrate. Example: Dehydrogenases are enzymes that remove hydrogen.

Induced-fit Theory

The shape of the enzyme must match the shape of the substrate. Enzymes are therefore very specific; they will only function correctly if the shape of the substrate matches the active site.

Enzyme and substrates
An enzyme-substrate complex forms when the enzyme’s active site binds with the substrate like a key fitting a lock.

Enzyme-substrate complex

The product is released from the enzyme. The enzyme can be reused.

Enzyme and product

The substrate molecule normally does not fit exactly in the active site. This induces a change in the enzymes conformation (shape) to make a closer fit.

In reactions that involve breaking bonds, the inexact fit puts stress on certain bonds of the substrate. This lowers the amount of energy needed to break them.

The enzyme does not form a chemical bond with the substrate. After the reaction, the products are released and the enzyme returns to its normal shape.

Because the enzyme does not form chemical bonds with the substrate, it remains unchanged. As a result, the enzyme molecule can be reused. Only a small amount of enzyme is needed because they can be used repeatedly.

Activation Energy and Enzymes

The amount of activation energy that is required is considerably less when enzyme is present.

Enzymes reduce activation energy.

Conditions that Affect Enzymatic Reactions

Rate of Reaction

Reactions with enzymes are up to 10 billion times faster than those without enzymes. Enzymes typically react with between 1 and 10,000 molecules per second.

Fast

You May Also Find These Documents Helpful

  • Better Essays

    GRT1 Task 4

    • 964 Words
    • 4 Pages

    Enzymes only react with substrates that are specific to that enzyme. When a substrate is accepted by the enzyme, the end result is a product. This product becomes the substrate for the next enzyme in the pathway.…

    • 964 Words
    • 4 Pages
    Better Essays
  • Satisfactory Essays

    2aii. The enzyme is denatured, this means that the substrate will no longer fit into the active site and is no longer complementary.…

    • 290 Words
    • 2 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Enzyme Lab Quiz

    • 293 Words
    • 2 Pages

    4. Enzyme-substrate complex – modifies the substrate’s chemical bonds and initiates a series of chemical reactions resulting in the formation of a product.…

    • 293 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    Apple sauce lab

    • 700 Words
    • 7 Pages

    enzymes, for enzymes such as pectinase. pectinase is an enzyme that breaks down pectin in…

    • 700 Words
    • 7 Pages
    Better Essays
  • Powerful Essays

    It is important to denature the enzyme because leaving it active would increase the activity of the enzyme.…

    • 1209 Words
    • 5 Pages
    Powerful Essays
  • Good Essays

    In this lab we explore an enzymes activity and how it can be affected by changes to its environment. An enzyme is a protein and is a catalyst to chemical reactions (Raven, 2011). It helps accelerate reactions by lowering the activation energy, which is needed for reactions in cells to progress at a higher rate (Kaiser, 2001). Activation energy is the minimum amount of energy needed for a chemical reaction to occur, yielding products from a given set of reactants. Products are results of an enzyme cleaving to a specific substrate, by means of an induced fit. The induced fit is located at the active site of the enzyme or region of the enzyme where the substrate is bound. The substrate is the reactant within the reaction that fits with the enzyme like a key into a lock. Once the substrate enters the enzyme’s active site the enzyme can flexibly change shape to more snugly bind, via the induced fit, to form an enzyme-substrate complex. The substrate is then metabolized or broken down, resulting in a product, which can be utilized to energize cells. Once the product is released from the active site the enzyme returns to…

    • 621 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    | * Specificity on substrates:Enzymes are specific in action and react with only one substrate. Due to the shape of the enzymes active site (where reactions occur and products are made)LOCK AND KEY-INDUCED FIT-…

    • 848 Words
    • 4 Pages
    Satisfactory Essays
  • Powerful Essays

    Catalase Lab

    • 1381 Words
    • 6 Pages

    Background: Enzymes are biological catalysts that carry out cellular metabolic processes with the ability to enhance the rate of reaction between. They are large proteins made up of several hundred chains of amino acid. In an enzyme-catalyzed reaction, the substance to be acted upon, or substrate, binds to the active site of the enzyme. The enzyme and substrate are held together…

    • 1381 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    Bio Lab

    • 573 Words
    • 3 Pages

    Enzymes are biological catalysts that speed up the process of chemical reactions. They are also proteins, and most enzymes activities occur within organism. They decrease activation energy, energy that is needed to start a chemical reaction. Enzymes are substrate specific substrates ending in "-ase", enzymes ending in "-ase". External factors, such as temperature, pH, and concentration of the substrate, affect the enzymes activity in the lab, pectase and cellulase will be used to compare different enzymes on apple sauce.…

    • 573 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Peroxidase Experiment

    • 1061 Words
    • 5 Pages

    An enzyme is typically a protein with a specific three-dimensional shape. As previously mentioned above a small part of this shape forms the active site, where the enzyme combines with the substrate. The substrate actually fits into the active site, which is why enzymes are specific to the reaction they catalyze. (Campbell, N,…

    • 1061 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Biology Hsc Notes

    • 7966 Words
    • 32 Pages

    * They are highly specific in their actions; this means that each enzyme acts on one substrate only…

    • 7966 Words
    • 32 Pages
    Good Essays
  • Good Essays

    As you can see, when an enzyme is added to a reaction, it reacts with the substrate but also comes back out at the end, it never changes.…

    • 1747 Words
    • 7 Pages
    Good Essays
  • Powerful Essays

    Review for Essay Tests

    • 1211 Words
    • 5 Pages

    An enzyme is a complex protein containing one or more polypeptide chains. Enzymes form the globular shape crucial for enzyme-substrate interactions. The active site of the enzyme is the portion of the enzyme that fits over the molecule. The substrate fits into the active site to form the enzyme-substrate complex. The factors that modify enzyme structure and/or function include temperature and substrate concentration. The substrate concentration can modify the enzyme because the reaction is dependent on the concentration of the enzyme, substrate, and product. If there is an abundance of substrate and a subsequent lack of product, the reaction will form more product, and vice versa with more substrate being formed in an abundance of product. The temperature modifies the enzyme’s structure and/or function because if the temp increases, the rate of reaction increase, and if it decreases so does the rate of reaction.…

    • 1211 Words
    • 5 Pages
    Powerful Essays
  • Better Essays

    Renin experiment

    • 2887 Words
    • 12 Pages

    An enzyme works in very complex ways almost like the way a key fits into a lock. When a certain molecule which holds the right chemical compound passes the enzyme it will attach itself. (See figure 1) The area in…

    • 2887 Words
    • 12 Pages
    Better Essays
  • Powerful Essays

    Biology

    • 39898 Words
    • 160 Pages

    • Enzymes are globular proteins whose shapes are specialised so that other chemicals (substrates) can form a temporary bond with them. There are two models used to show how an enzyme work:…

    • 39898 Words
    • 160 Pages
    Powerful Essays