Lab Report Essay Example
Kimmi Dodia- Biomedical Science- 1019971
Lab partner- Kim Harcourt
Lab report- Experiment 1: Microbial Genetics
Abstract
The objective of this experiment was to introduce the study of bacterial genetics in order to identify the potency of different mutagenic agents. Our primary aim was to demonstrate the different techniques needed to isolate biosynthetic auxotrophic mutants using chemical, physical and transposon mutagenesis. The second aim was to plan and execute an experiment designed to isolate catabolic mutants (Lac-) as well as conditional mutants (Temperature sensitive) using only a chemical treatment (EMS). Also, to make and characterize transposon-insertion mutants of KL14 using bacteriophage as a transposon delivery vector. To identify and demonstrate the techniques needed to enrich and isolate the biosynthetic auxotrophic, catabolic auxotrophic and conditional mutants. Lastly, we were to characterize biosynthetic auxotrophic mutants and effectively characterize itʼs biosynthetic pathway. We determined that the UV light treatment was the most potent treatment of mutagenesis and also the most effective in inducing mutations, followed by the Tn5 and the EMS was the least potent and effective treatment.
Introduction
A culture of E.coli K12, KL14 was used to carry out the experiment on. The advantage of using E.coli cells to experiment on is that they are haploid organisms and contain a single set of chromosomes so if a mutation was to occur, the bacterial cell will express it as there is only one copy of the gene. We experimented on various types of mutations. Auxotrophic mutants are impaired in their metabolic capabilities, they can either be biosynthetic auxotrophs which require an additional nutrient in the minimal media in order to grow or can be catabolic auxotrophs, which are affected in their ability to utilize a particular substance for growth. The phenotype of a conditional mutant is expressed only when the organism is grown under a
Lab partner- Kim Harcourt
Lab report- Experiment 1: Microbial Genetics
Abstract
The objective of this experiment was to introduce the study of bacterial genetics in order to identify the potency of different mutagenic agents. Our primary aim was to demonstrate the different techniques needed to isolate biosynthetic auxotrophic mutants using chemical, physical and transposon mutagenesis. The second aim was to plan and execute an experiment designed to isolate catabolic mutants (Lac-) as well as conditional mutants (Temperature sensitive) using only a chemical treatment (EMS). Also, to make and characterize transposon-insertion mutants of KL14 using bacteriophage as a transposon delivery vector. To identify and demonstrate the techniques needed to enrich and isolate the biosynthetic auxotrophic, catabolic auxotrophic and conditional mutants. Lastly, we were to characterize biosynthetic auxotrophic mutants and effectively characterize itʼs biosynthetic pathway. We determined that the UV light treatment was the most potent treatment of mutagenesis and also the most effective in inducing mutations, followed by the Tn5 and the EMS was the least potent and effective treatment.
Introduction
A culture of E.coli K12, KL14 was used to carry out the experiment on. The advantage of using E.coli cells to experiment on is that they are haploid organisms and contain a single set of chromosomes so if a mutation was to occur, the bacterial cell will express it as there is only one copy of the gene. We experimented on various types of mutations. Auxotrophic mutants are impaired in their metabolic capabilities, they can either be biosynthetic auxotrophs which require an additional nutrient in the minimal media in order to grow or can be catabolic auxotrophs, which are affected in their ability to utilize a particular substance for growth. The phenotype of a conditional mutant is expressed only when the organism is grown under a