AP Lab 4
Determining the rate of Cellular Respiration Using Germinating and Dry Peas
Purpose: To determine the rate of cellular respiration using dry and germinating peas.
Introduction: In this lab, we are investing cellular respiration, specifically aerobic cellular respiration. Aerobic cellular respiration is the process by which cells consume oxygen during the oxidation of glucose and produce CO2 as a byproduct. During cellular respiration, glucose is split into a 6 carbon sugar, G3P through the use of 2 ATP, and then G3P is oxidized to form a 3 carbon molecule, pyruvate. This process is known as glycolosis. After glycolosis, pyruvate enters the mitochondria and is converted into Acetyl CoA. This is done because pyruvate’s carboxyl group is oxidized and given off as CO2 and the remaining pyruvate is oxidized to form acetate. Coenzyme A is then attached to the acetate through the help of sulfur. Acetyl CoA then enters the Citric Acid Cycle where NADH and FADH2 are produced and enter the ETC where oxygen, the final electron acceptor, oxidizes NADH and FADH2 to form water. This equation: C6H12O6 + 6 O2-------→6 CO2 + H2O shows how glucose is oxidized and the different ways of measuring cellular respiration. For this lab, we will be measuring the amount of oxygen consumed by using a respirometer, where the amount of oxygen consumed will be measured by the amount of water taken into the respirometer.
Hypothesis: 1. If I place the 3-day germinating peas in the respirometer, then there will be a high amount of oxygen consumption and a faster rate of oxygen consumption in both temperatures. 2. If I place the dry peas and beads in the respirometer, then there will a low amount of oxygen consumption and low rate of oxygen consumption in both temperatures. 3. If I place the glass beads in the respirometer, then there will be no oxygen consumption.
• 3 respirometers
• Germinating peas
• Dry peas
• Glass beads