Bio11

Topics: DNA / Pages: 20 (1424 words) / Published: Apr 20th, 2014
APPROVED

ACADEMIC SESSION:

2011/2012

Campus

MEDWAY

School

School of Science

Level

Four

Date

May 2012

COURSE CODE

BIOC1011

COURSE TITLE

FUNDAMENTALS OF BIOCHEMISTRY

Duration

2 Hours

___________________________________________________________________________
INSTRUCTIONS TO CANDIDATES

SECTION A
Answer all the multiple choice questions on the answer sheet provided.
Each question has ONE correct answer only.
Each question is worth one mark

SECTION B
Short essay questions.
Answer TWO questions only (5 questions provided).
Each question is worth 33 marks.

Date
Course Title
Course Code

May 2012
Fundamentals of Biochemistry
BIOC1011
Page 1 of 8

APPROVED
SECTION A
Answer all the multiple choice questions on the answer sheet provided. Each question has
ONE correct answer only.
1.

In DNA amplification:
a)
b)
c)
d)
e)

2.

In gene cloning what function do ligase enzymes perform?
a)
b)
c)
d)
e)

3.

A PCR-amplified fragment
A single stranded RNA hybridized to a single stranded DNA
A genomic fragment of Human DNA ligated to a bacterial plasmid vector
A bacterial plasmid cut with a restriction enzyme
None of the above

A genomic library is:
a)
b)
c)
d)
e)

6.

Type II restriction enzymes are used in gene cloning research
Restriction enzymes cut methylated DNA
Restriction enzymes can produce DNA molecules with blunt or sticky ends
Short restriction sites occur more frequently in the genome
None of the above

Which of the following is an example of a recombinant DNA molecule?
a)
b)
c)
d)
e)

5.

Cutting of DNA at specific sites
Adding chemical groups to DNA
Unwinding supercoiled DNA
Joining nucleic acids together covalently
None of the above

Which of the following statements is FALSE?
a)
b)
c)
d)
e)

4.

the annealing reaction occurs at about 70oC the denaturation reaction occurs at about 50oC the extension reaction occurs at about

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