Bio11

Topics: DNA, Molecular biology, Enzyme Pages: 19 (1424 words) Published: April 20, 2014
APPROVED

ACADEMIC SESSION:

2011/2012

Campus

MEDWAY

School

School of Science

Level

Four

Date

May 2012

COURSE CODE

BIOC1011

COURSE TITLE

FUNDAMENTALS OF BIOCHEMISTRY

Duration

2 Hours

___________________________________________________________________________ INSTRUCTIONS TO CANDIDATES

SECTION A
Answer all the multiple choice questions on the answer sheet provided. Each question has ONE correct answer only.
Each question is worth one mark

SECTION B
Short essay questions.
Answer TWO questions only (5 questions provided).
Each question is worth 33 marks.

Date
Course Title
Course Code

May 2012
Fundamentals of Biochemistry
BIOC1011
Page 1 of 8

APPROVED
SECTION A
Answer all the multiple choice questions on the answer sheet provided. Each question has ONE correct answer only.
1.

In DNA amplification:
a)
b)
c)
d)
e)

2.

In gene cloning what function do ligase enzymes perform?
a)
b)
c)
d)
e)

3.

A PCR-amplified fragment
A single stranded RNA hybridized to a single stranded DNA
A genomic fragment of Human DNA ligated to a bacterial plasmid vector A bacterial plasmid cut with a restriction enzyme
None of the above

A genomic library is:
a)
b)
c)
d)
e)

6.

Type II restriction enzymes are used in gene cloning research Restriction enzymes cut methylated DNA
Restriction enzymes can produce DNA molecules with blunt or sticky ends Short restriction sites occur more frequently in the genome
None of the above

Which of the following is an example of a recombinant DNA molecule? a)
b)
c)
d)
e)

5.

Cutting of DNA at specific sites
Adding chemical groups to DNA
Unwinding supercoiled DNA
Joining nucleic acids together covalently
None of the above

Which of the following statements is FALSE?
a)
b)
c)
d)
e)

4.

the annealing reaction occurs at about 70oC
the denaturation reaction occurs at about 50oC
the extension reaction occurs at about 70oC
the extension reaction occurs at about 95oC
None of the above is true

A collection of cloned restriction enzyme digested DNAs containing at least 1 copy of every DNA sequence in the genome
A collection of cloned restriction enzyme digested fragments from individual chromosomes
A collection of clones of DNA copies made from mRNA isolated from cells All of the above
None of the above

PCR CANNOT be successfully performed without:
a)
b)
c)
d)
e)

At least 100 starting molecules
At least some sequence information about the region to be amplified A cDNA version of the region to be amplified
A section of at least 100Kb to amplify
All of the above
Date
May 2012
Course Title
Fundamentals of Biochemistry
Course Code
BIOC1011
Page 2 of 8

APPROVED
7.

In gel electrophoresis of DNA, fragments move at different rates because they have different:
a)
Charge densities
b)
Sizes
c)
Base sequences
d)
Amino acid compositions
e)
Electrical strengths

8.

Stringency of hybridization is increased by:
a)
b)
c)
d)
d)

Increasing the salt concentration and reducing the temperature Reducing the salt concentration and reducing the temperature Reducing the salt concentration and increasing the temperature Increasing the salt concentration and increasing the temperature None of the above

9.

What information CANNOT be determined from the DNA sequence of a cDNA clone?
a)
Exon sequences
b)
Sequence of the promoter
b)
Similarity to previously identified sequences
c)
Amino acid sequence of the encoded polypeptide
d)
Sequence of the spliced mRNA transcript

10.

Northern blotting can be used to estimate quantities of:
a)
b)
c)
c)
d)

11.

Southern blotting is used to detect:
a)
b)
c)
d)
e)

12.

A specific polypeptide
Restriction fragments of DNA
A specific mRNA
Vector DNA
None of the above

DNA
RNA
mRNA
Protein
All of the above

Enzymes that catalyse a reaction:
a)
b)
c)
d)
e)

Date
Course...
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