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Bio Objectives for Exam 2

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Bio Objectives for Exam 2
Objectives for Lecture 9
Know the difference in resolution and magnification between light and electron microscopes.
Understand the process of cell fractionation based on centrifugation and know what the purpose of cell fractionation is.
Know what the differences in cell structure are between prokaryotic cells and eukaryotic cells
Know that most eukaryotic cells are between 10-100 m in diameter, whereas most prokaryotic cells are about 1 m in diameter.
Know the following terms, plasma membrane, cytoplasm, nucleus, cell wall, ribosomes, chromosomes.
Know the general structure and function are of the following cellular structures and organelles: plasma membrane, nucleus, ribosomes, ER (rough and smooth), Golgi, lysosomes, mitochondria, and chloroplasts. Be able to identify each of these organelles in photographs or drawings of cells.
Understand that the pathway for the flow of materials to the cell surface is from ER to the Golgi, to Golgi vesicles, to the plasma membrane
Know that cell shape and cellular movements are mediated by the cytoskeleton, which is composed of microtubules (tubulin protein), microfilaments (actin protein), and intermediate filaments (keratin protein)
Know the major structural differences between animal and plant cells.

Lecture 10
Be able to describe the structure of biological membranes (lipid bilayers + membrane proteins).
Be able to list the functions of membrane proteins (transport, surface for chemical reactions, hormone perception, cell-to-cell attachment, cell-cell recognition, attachment points of cytoskeleton and extracellular proteins to the membrane).
Know that biological membranes are semipermeable (more permeable to some solutes than others) and know how the size, polarity, and charge affects the permeability of solutes through membranes.
Understand the terms passive transport and diffusion, and osmosis.
Be able to make predictions about the direction that solutes or water will move if given

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