bio 260
Briefly define aseptic technique.
Why is it necessary to heat a loop to redness when flamed? Why is it necessary to cool a loop after it has been flamed BEFORE placing it into a bacterial culture? What are three qualities of an effective and useful bacterial smear? What would happen to a smear if you failed to heat fix the slide before staining? Differentiate between a differential stain and a special stain (how are they different, don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure if the alcohol step in the gram stain was omitted? What color would Gram negative bacteria be after the alcohol wash before the counter-stain? In performing the spore stain, why is it necessary to heat the malachite green? When performing the Endospore stain, what color do the bacteria appear at the end of the stain? Are the bacteria the same color as the endospore, briefly explains?
Briefly define aseptic technique. Why is it necessary to heat a loop to redness when flamed? Why is it necessary to cool a loop after it has been flamed BEFORE placing it into a bacterial culture? What are three qualities of an effective and useful bacterial smear? What would happen to a smear if you failed to heat fix the slide before staining? Differentiate between a differential stain and a special stain (how are they different, don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the
Why is it necessary to heat a loop to redness when flamed? Why is it necessary to cool a loop after it has been flamed BEFORE placing it into a bacterial culture? What are three qualities of an effective and useful bacterial smear? What would happen to a smear if you failed to heat fix the slide before staining? Differentiate between a differential stain and a special stain (how are they different, don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the role of the alcohol wash in the Gram Stain? What color would all bacteria become at the end of the staining procedure if the alcohol step in the gram stain was omitted? What color would Gram negative bacteria be after the alcohol wash before the counter-stain? In performing the spore stain, why is it necessary to heat the malachite green? When performing the Endospore stain, what color do the bacteria appear at the end of the stain? Are the bacteria the same color as the endospore, briefly explains?
Briefly define aseptic technique. Why is it necessary to heat a loop to redness when flamed? Why is it necessary to cool a loop after it has been flamed BEFORE placing it into a bacterial culture? What are three qualities of an effective and useful bacterial smear? What would happen to a smear if you failed to heat fix the slide before staining? Differentiate between a differential stain and a special stain (how are they different, don’t simply identify the types of differential stains and special stains). What is the role of the Gram’s iodine in a Gram stain? What color would all bacteria become at the end of the staining procedure if the iodine in the gram step was omitted? What is the