Zebrafish Embryotoxiration Test Lab Report
Zebrafish Embryotoxicity Test (ZET)
The ZET will be done following the method proposed by Beekhuijzen et al. (2015) with modifications. Six embryos will be exposed to each of the following treatments: 0.1, 0.05, 0.025, 0.0125, and 0.00625 mg/ml. Embryos will be incubated in 24-well plates with built-in lids. Each well will contain one embryo and 2 ml of the respective treatment. All test solutions will be renewed every 24h to maintain the integrity of the test concentrations.
The embryos will be incubated in an air-conditioned room at 26ºC as an alternative to the unavailability of an incubator (OECD, 2013). During the test, the embryos will be exposed to white light in a 12h: 12h light/dark cycle (Villamizar, Vera, Foulkes, & Sánchez-Vázquez, 2014) to ensure the greatest survival …show more content…
Five concentrations of TE will be tested, the highest of which will be the highest concentration revealed by the embryotoxicity assay as non-lethal and non-teratogenic. The other concentrations will be determined by using 2 as a spacing factor.
Six embryos will be exposed to each of the test concentrations in 24-well plates. The embryos will be incubated at 26 ºC for 48 h in a 12h/12h light-dark cycle in white light. After which, the embryos will be rinsed in aged system water, and exposed to 2% ethanol for 48h. All test solutions will be renewed every 24 h. A 24-h extension of the exposure period done by Arnold et al. (2015) will be needed to allow the researcher to use the teratogenicity scoring system from Beekhuijzen et al. (2015) to assess until the 96th hour of exposure, corresponding to the 48th hour of exposure in the antiteratogenicity assay, the potential of TE to rescue embryos from
The ZET will be done following the method proposed by Beekhuijzen et al. (2015) with modifications. Six embryos will be exposed to each of the following treatments: 0.1, 0.05, 0.025, 0.0125, and 0.00625 mg/ml. Embryos will be incubated in 24-well plates with built-in lids. Each well will contain one embryo and 2 ml of the respective treatment. All test solutions will be renewed every 24h to maintain the integrity of the test concentrations.
The embryos will be incubated in an air-conditioned room at 26ºC as an alternative to the unavailability of an incubator (OECD, 2013). During the test, the embryos will be exposed to white light in a 12h: 12h light/dark cycle (Villamizar, Vera, Foulkes, & Sánchez-Vázquez, 2014) to ensure the greatest survival …show more content…
Five concentrations of TE will be tested, the highest of which will be the highest concentration revealed by the embryotoxicity assay as non-lethal and non-teratogenic. The other concentrations will be determined by using 2 as a spacing factor.
Six embryos will be exposed to each of the test concentrations in 24-well plates. The embryos will be incubated at 26 ºC for 48 h in a 12h/12h light-dark cycle in white light. After which, the embryos will be rinsed in aged system water, and exposed to 2% ethanol for 48h. All test solutions will be renewed every 24 h. A 24-h extension of the exposure period done by Arnold et al. (2015) will be needed to allow the researcher to use the teratogenicity scoring system from Beekhuijzen et al. (2015) to assess until the 96th hour of exposure, corresponding to the 48th hour of exposure in the antiteratogenicity assay, the potential of TE to rescue embryos from