Preview

Yeast Coorperation

Satisfactory Essays
Open Document
Open Document
264 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Yeast Coorperation
Ratidzai Lyanne Mapani

1000040207

PRA 0402

Citation

Harrison E, Koufopanou V, Burt A, MacLean RC. 2012. The cost of copy number in a selfish genetic element: the 2-μm plasmid of Saccharomyces cerevisiae. J Evol Biol 25(11): 2348-56.

What are the actors (e.g., what parts of the organism are in conflict) and what are they in conflict over?

The 2 μm plasmid of Saccharomyces yeast is in conflict with the cell host, this plasmid cost the host through using the cells' resources ; meaning a burden on the host to synthesize more proteins as well as increasing the amount of DNA the host cells needs to accommodate.

What is the key result of this experiment or study?

The results of the experiment come to prove that the higher plasmid copy numbers of 2 μm come at a consequence of a greater fitness cost to the host cell, seen through a decrease in growth rate.

What circumstances do you think would reduce the genetic conflict described in the article?

Since this genetic conflict occurs as a result of a trade-off between selection for higher vertical transmission which favours higher plasmid copy number and selection for lowering fitness cost to the host which would favour reduced copy number, this genetic conflict would be reduced if the later where of greater value. This could be done by reducing the efficiency of vertical transmission between the plasmids to maximize the rate of plasmid loss during asexual growth.

Does this genetic conflict involve a selfish genetic element?

This genetic conflict involves Selfish Genetic Elements

You May Also Find These Documents Helpful

  • Good Essays

    FinalExam5HW

    • 2867 Words
    • 9 Pages

    -A Plasmid is a self-replicating circle of DNA that has a few genes and can be passed from one cell to another.…

    • 2867 Words
    • 9 Pages
    Good Essays
  • Good Essays

    D) cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of…

    • 4889 Words
    • 20 Pages
    Good Essays
  • Good Essays

    The topic of this research involved the occurrence of genetic transformation in bacteria (E. Coli). More specifically, a previously prepared pGLO plasmid--which consisted of the gene to be cloned--was used to transform non-pathogenic bacteria. The pGLO plasmid contained a gene for the Green Fluorescent Protein (GFP) from a bioluminescent jellyfish and a gene for resistance to ampicillin, an antibiotic. Essentially, we wanted to determine the conditions of the bacteria that would glow. Our hypothesis was that the transformed solution with no plasmid DNA and ampicillin would produce no bacteria colonies, as it wouldn 't be able to grow without the gene for ampicillin resistance. Also, the transformed solution with just LB and ampicillin would produce bacteria colonies but the transformed solution with LB/ampicillin/Arabinose would produce glowing bacteria colonies (as Arabinose allows the GFP gene to be expressed, but in both cases bacteria colonies would be present because of the gene of resistance to the antibiotic, ampicillin). We essentially made the required transformed solutions--and the controls--swiped them on the agar plate, and then observed to see whether or not bacteria colonies grew and whether or not they glowed. Our data fully supported our hypothesis. We can thus conclude that bacteria can take in foreign DNA through the process of transformation and that this foreign DNA can fundamentally change the bacteria (ex: making it glow). Future research can involve inserting other pieces of DNA into bacteria from different organisms, making the bacteria take on various phenotypic characteristics.…

    • 1330 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Cis499

    • 402 Words
    • 2 Pages

    A plasmid is a circular segment of DNA with an origin of replication. It can be duplicated and serve as an adjunct to the main bacterial chromosome.…

    • 402 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Plasmids are small circular autonomously replicating pieces of DNA that can be found inside of a prokaryotic bacterial cell. By barrowing a cell’s polymerase they replicate their own DNA. They are easy to extract from the bacterial cells due to their size. Plasmids are helpful for cloning foreign genes because of their ability to express antibiotic resistance as well their ability to be modified to express proteins of interest. A pGLO plasmid contains genes for the green florescent protein (GFP) as well as the gene for ampicillin resistance known as beta-lactamase. It also contains a gene regulation system (operon) that has the ability to control expression of the GFP gene in transformed cells known as araC. The source of GFP is naturally founds within a…

    • 463 Words
    • 2 Pages
    Good Essays
  • Better Essays

    4. Cut plasmid and eukaryotic DNA with the same restriction enzyme… Mix fragments and allow them to match… Ligate… Transform into bacteria… Identify desired clone.…

    • 1001 Words
    • 5 Pages
    Better Essays
  • Better Essays

    Biology Exam Q&a

    • 1892 Words
    • 8 Pages

    What is a plasmid? Answer: Self-replicating circle of DNA that has a few genes; Can be passed from one cell to another…

    • 1892 Words
    • 8 Pages
    Better Essays
  • Good Essays

    Plasmid: A extra chromosomal which may or may not integrate with the main genome. It gives additional characters to the cell like antibiotic resistance.…

    • 814 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Gene Therapy: Lab Report

    • 2285 Words
    • 8 Pages

    The purpose of this lab was to demonstrate the use of gene therapy on diseases that are caused by a single gene defect. This procedure was demonstrated on two different strains of baker’s yeast, EAY 235 and EAY 431, which both contained mutations in the LEU2 and TRP1 genes. Neither of these strains will grow without a proper medium that would supply both of these essential amino acids. The EAY 431 strain of yeast also contained a Rad 52 deletion, which caused EAY 431 to be a deficient, recombinant strain. The LEU2 gene is a linear fragment that does not contain an Autonomous Replication Sequence, so it could not replicate on its own and needed to be integrated by homologous recombination. The TRP1 gene was a circular plasmid that contained an ARS, which allowed for it to act as an extra chromosome in the gene. The objective was to insert a “wild gene” and replace the defective genes and then grow them on a medium that does not contain TRP1 or LEU2 to prove that the genes had been cured.…

    • 2285 Words
    • 8 Pages
    Powerful Essays
  • Good Essays

    Virology Quiz

    • 1824 Words
    • 8 Pages

    Early genes in most viruses are involved in : a. Regulating host functions b. Making RNA polymerases c. Replicating the virus genome d. Virus assembly e. Virus release 46. Genome entry for bacteriophage T7 is due to : a. Transcription by host enzymes b. Transcription by viral enzymes c. Injection d. Pilus retraction e. A and B 47. Why is the initial amount of DNA injected into a host cell on infection by bacteriophage T7 so short? a. So that the host RNA polymerase can bind its promoters b. So that the viral DNA is not degraded by host endonucleases c. So that the viral DNA is not degraded by viral endonucleases d. Because the DNA in the virion is not tightly packed e. So that the viral RNA polymerase can be made 48. Why is it thought that T7 lysozyme inhibits T7 RNA polymerase activity? a. Because T7 RNAP is too fast b. Because the genome is already in the cell c. Because T7 RNAP activity is not required late in infection d. To allow the holin protein to be made e. To serve as a scaffold for virion assembly 49. T7 DNA replication resembles host replication except : a. The requirement for a helicase b. That there is only one replication fork c. That it is “rolling circle” d. That single strands are made e. For the proteins involved 50. Most modern plasmid vectors contain : a. PhiX174 origins of replication b. Podovirus RNA polymerase promoters c. T7 DNA polymerase origins d. T7 holin genes e. All of the…

    • 1824 Words
    • 8 Pages
    Good Essays
  • Powerful Essays

    Biology Lab

    • 2372 Words
    • 10 Pages

    The variables were kept controlled by using different inoculating loops to add and spread out each solution. This was done to prevent any solutions from mixing together. The same amount of pGLO plasmid solution was added by dipping the loop until a bubble was formed across the loop opening. The tubes were incubated for the same amount of time and heat shocked for the same amount of time. The same amounts of Luria broth and cell suspension were used to keep the number of individual colonies controlled.…

    • 2372 Words
    • 10 Pages
    Powerful Essays
  • Satisfactory Essays

    Burro Genius

    • 13815 Words
    • 56 Pages

    Lartigue, Carol, et al. “Genome Transplantation in Bacteria: Changing One Species to Another.” Science 317 (2007): 632–38.…

    • 13815 Words
    • 56 Pages
    Satisfactory Essays
  • Powerful Essays

    The book focuses on selfishness being passed on through the gene and individual animal level rather than the group level. A whole gene is usually not passed down through generations. A miniscule part of a gene/chromosome can be passed down through many generations. The smaller the gene, the more likely it is to passed through many generations. The gene is not exactly a physical piece of DNA, but rather a primeval soup that has replicas all around the world.…

    • 709 Words
    • 3 Pages
    Powerful Essays
  • Powerful Essays

    In this experiment, we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis, we analyzed the gel photograph by using a standard DNA marker, Lambda HindIII, and came to a conclusion based on our results.…

    • 3383 Words
    • 14 Pages
    Powerful Essays
  • Good Essays

    Gene Transfer Lab Report

    • 939 Words
    • 4 Pages

    The following experiment method is based on the procedure given through the Biology Department at UWM (Wimpee, 2006). This experiments started with two tubes of 100 uL E. coli cells, labeled one and two. Tube one just contained normal E. coli cells. Tube two was the tube with the plasmid added to it. The first step in this experiment was to add plasmid DNA, the “mini chromosomes” of the bacteria, to the E. coli cells in order to change the genetic makeup of them. I then added 10 uL of the plasmid to tube two. The next step was to chill both the tubes E.…

    • 939 Words
    • 4 Pages
    Good Essays

Related Topics