Worksheet

Worksheet
Fill in the following tables:
Type of
Microscopy

State of the
Cell
(live, dead, both) Limit of General steps for resolution sample preparation/ fixation

Advantage of
Technique

Phase contrast light microscope
Amplitude
contrast microscope Optical tweezers Fluorescence/
Confocal
Microscopy
Transmission
Electron
Microscope
(TEM)
Scanning
Electron
Microscope
(SEM)
Scientist
Robert Hooke
Antonie van Leeuwenhoek
Ernst Karl Abbe
Fritz Zernike
Shimomura, Chalfie, and Tsien
Ernst Ruska
Schleiden and Schwann
Buckminster Fuller

Contribution

Disadvantages of
Technique

Define the following:
The Cell:
Optical tweezers:
Fluorochrome:
Electron Tomography:
Centrifugation:
Differential Centrifugation:
Equilibrium Sedimentation:
Homogenate:
Electrophoresis:
Column Chromatography:
Isoelectric Focusing:
Western Blotting:
Flow Cytometry:
2-D surface metabolism:
Tensegrity:

Answers the following questions:


What is the difference between Resolution and Magnification?



What is the difference between Amplitude contrast and Phase contrast?



A student in a cellular biology laboratory is faced with the task of detecting protein X in muscle cells taken from a rat. The tissue is required to be live while under analysis. Which type of microscopy should be employed?
a) Phase contrast light microscopy
b) Transmission electron microscopy
c) Scanning electron microscopy
d) Fluorescence microscopy
e) None of the above



Which of the following about the Cell Theory is FALSE?
a) All cells come from preexisting cells through division
b) All organisms consist of one or more cells
c) The cell is the basic unit of structure for all organisms
d) All cells must contain organelles
e) None of the above



This organelle is involved in the synthesis of lipids, the detoxification of drugs and toxins, and in storage of ions.
a) Rough endoplasmic reticulum
b) Smooth endoplasmic reticulum
c) Mitochondria
d) Golgi apparatus
e) Nucleus



Which of the following is NOT found in the