Withania Somnifera Case Study
This study was aimed to evaluate the Protective effect of Withania somnifera on forced swimming induced CFS (Chronic Fatigue Syndrome) in male albino rats. Withania somnifera i.e., Ashwagandha churna, an ayurvedic herbal drug was used in this study. Forced swimming for 15 min for consecutively for a period of 21 days was used to induce the CFS. Imipramine was selected as standard drug. Several parameters like Behavioural (Imobility, Anxiety, Locomotor activity) and Biochemical (Lipid peroxidation, catalase, and Superoxide desmutase) parameters were used to evaluate the activity. Two different doses of Ashwagandha i.e., 360 and 720 mg/kg body weight and 20mg/kg body weight of Imipramine as standard drug were used to evaluate their protective
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Major symptoms of CFS are Impaired memory or concentration, unrefreshing sleep, myalgia, arthralgia, tender lymph nodes (cervical or axillary). The pathophysiology of chronic fatigue syndrome is unknown. Several potential causes for the development of chronic fatigue syndrome have been proposed, including neurological factors, psychological or psychosocial factors or influences, infections, immunological factors, endocrinal factors and genetic factors. Other, less-common theories have also been articulated. No clinically meaningful risk factor has been identified.2 Treatment of chronic fatigue syndrome (CFS) is variable and uncertain, and the condition is primarily managed rather than cured.3 Even when treated, the prognosis of CFS is often poor.4 Malondialdehyde, a product of lipid peroxidation, was found to be significantly higher in CFS patients. Peroxidation of erythrocyte membrane lipid in CFS is also a strong evidence that suggests’ that free radicals play a part in the pathogenesis of this …show more content…
Each animal was placed individually in the actophotometer for 3 min as a habituation period and the locomotor activity was recorded for the next 5 minutes. The ambulatory activity was recorded and expressed in terms of total photo beam counts for 5 min per animal and was assessed on day 1, day 7, DAY 14 and day 21 in the naïve, stress control, stress with WS Dose I, and stressed with WS Dose II and standard drug with stress groups.
Assessment oxidative stress
Dissection and Homogenization
24hours after the last forced swimming, experimental animals were sacrificed by decapitation. The whole brain was removed and a 10% (w/v) tissue homogenate was prepared in 0.1 M phosphate buffer (pH 7.4). The post nuclear fraction obtained by centrifugation of the homogenate at 1000 rpm for 15 min. was used for catalase assay. For other enzyme assays, the homogenate were centrifuged at 10,000 rpm for 20 min.
1. Protein Estimation: The amount of protein was measured according to the method of Lowry et al. modified by Pomory, using bovine serum albumin as standard11.
2. Assessment of lipid Peroxidation: Malodialdehyde (MDA) levels were measured by the double heating
Major symptoms of CFS are Impaired memory or concentration, unrefreshing sleep, myalgia, arthralgia, tender lymph nodes (cervical or axillary). The pathophysiology of chronic fatigue syndrome is unknown. Several potential causes for the development of chronic fatigue syndrome have been proposed, including neurological factors, psychological or psychosocial factors or influences, infections, immunological factors, endocrinal factors and genetic factors. Other, less-common theories have also been articulated. No clinically meaningful risk factor has been identified.2 Treatment of chronic fatigue syndrome (CFS) is variable and uncertain, and the condition is primarily managed rather than cured.3 Even when treated, the prognosis of CFS is often poor.4 Malondialdehyde, a product of lipid peroxidation, was found to be significantly higher in CFS patients. Peroxidation of erythrocyte membrane lipid in CFS is also a strong evidence that suggests’ that free radicals play a part in the pathogenesis of this …show more content…
Each animal was placed individually in the actophotometer for 3 min as a habituation period and the locomotor activity was recorded for the next 5 minutes. The ambulatory activity was recorded and expressed in terms of total photo beam counts for 5 min per animal and was assessed on day 1, day 7, DAY 14 and day 21 in the naïve, stress control, stress with WS Dose I, and stressed with WS Dose II and standard drug with stress groups.
Assessment oxidative stress
Dissection and Homogenization
24hours after the last forced swimming, experimental animals were sacrificed by decapitation. The whole brain was removed and a 10% (w/v) tissue homogenate was prepared in 0.1 M phosphate buffer (pH 7.4). The post nuclear fraction obtained by centrifugation of the homogenate at 1000 rpm for 15 min. was used for catalase assay. For other enzyme assays, the homogenate were centrifuged at 10,000 rpm for 20 min.
1. Protein Estimation: The amount of protein was measured according to the method of Lowry et al. modified by Pomory, using bovine serum albumin as standard11.
2. Assessment of lipid Peroxidation: Malodialdehyde (MDA) levels were measured by the double heating