Introduction:
In 1909, Professor Morgan studied genetic change with Drosophila melanogaster, or fruit flies. Because of him, fruit flies have become popular for genetic research. Since fruit flies have a high reproductive rate and short life cycles, they are ideal for the study of genetics. The generation takes about 10-15 days to be complete depending on the temperature. Ideal temperatures range from 20-25°C, where 25° reduces the days it takes for an egg to turn into a larva. However, exposure to temperatures significantly higher or lower may result in death or imparities. There are several ways to distinguish the sexes. Females are generally larger than males. Females have seven segments in …show more content…
Vitals of the flies should be ordered from a biological company.
2. Use fly nap to put flies to sleep.
3. After a few minutes, these flies should be asleep. Remove them from the vital into a petri dish. With a soft brush, separate the boys from the girls.
4. Place and label males into a new vital with fly food.
5. Kill the remaining female flies by placing them in alcohol. These are assumed to be non-virgins, which are needless in this experiment.
6. Within the next few days, observe the larva that are left in the original vital. When flies emerge, use fly nap and separate the sexes again in a petri dish.
7. The females should be virgins if they were carefully observed and separated 10-12 hours after pupation. These females are kept and placed and labeled in a new vital with fly food.
8. The males can be placed in the vital from step 4.
9. The result should have 4 vitals. A virgin female and male vital for ebony and a virgin female and male vital for wild type. These are the F1 Generation that will be used to create the F2 Generation.
F2 Generation:
1. Cross opposite sex of ebony and wild