Why Is Preparative Gas Chromatography Used To Separate Volatile Compounds
Extraction
Important Peaks
Concluded Compound
C-5 #1
-CH, C=O cyclopentanone C-5 #2
-OH, -CH
1-hexanol
D-4 #1,2
C=O, -CH
Cannot conclude
Preparative Gas Chromatography was used to separate volatile compounds, which can then be identified. The injected sample becomes vaporized and was carried through the mobile column. It is usually carried by an inert gas. The gases were moved through the stationary column. The polarity of the components to the column would cause the separation of the components. Opposite to a TLC, if the stationary column was very polar, the more polar components will fall behind the non-polar component because of the stronger interaction with the column. So the component with the shorter retention time is non-polar, and a longer retention time means more polar, in …show more content…
This separates the components, and allows the detector to analyze each components independently of the other. Several factors change the separation of the components:boiling point and vapor pressure, polarity, column temperature, carrier gas flow rate, and column length. If the compound has a very low boiling point and a high vapor pressure, then this created a shorter retention time because the components were in the gas phase for a longer time. For polarity of column, the more similar the column and the compound, hypothetically polar, would create a much longer retention time and a shorter retention time for the non polar compound. If the temperature of the column was increased, the rate of movement was increased as well which decreases the retention time. The carrier gas flow rate also changed the separation. If the rate is increased, the components are moved through the column faster. However, if the temperature and the gas flow rate were too high, a poor separation occurs because the components aren't interacting with the column very long which leads to a faulty separation. Lastly, column length could have an effect on separation.
Important Peaks
Concluded Compound
C-5 #1
-CH, C=O cyclopentanone C-5 #2
-OH, -CH
1-hexanol
D-4 #1,2
C=O, -CH
Cannot conclude
Preparative Gas Chromatography was used to separate volatile compounds, which can then be identified. The injected sample becomes vaporized and was carried through the mobile column. It is usually carried by an inert gas. The gases were moved through the stationary column. The polarity of the components to the column would cause the separation of the components. Opposite to a TLC, if the stationary column was very polar, the more polar components will fall behind the non-polar component because of the stronger interaction with the column. So the component with the shorter retention time is non-polar, and a longer retention time means more polar, in …show more content…
This separates the components, and allows the detector to analyze each components independently of the other. Several factors change the separation of the components:boiling point and vapor pressure, polarity, column temperature, carrier gas flow rate, and column length. If the compound has a very low boiling point and a high vapor pressure, then this created a shorter retention time because the components were in the gas phase for a longer time. For polarity of column, the more similar the column and the compound, hypothetically polar, would create a much longer retention time and a shorter retention time for the non polar compound. If the temperature of the column was increased, the rate of movement was increased as well which decreases the retention time. The carrier gas flow rate also changed the separation. If the rate is increased, the components are moved through the column faster. However, if the temperature and the gas flow rate were too high, a poor separation occurs because the components aren't interacting with the column very long which leads to a faulty separation. Lastly, column length could have an effect on separation.