Sheran Gu
Meghan Lena
Nov. 15th
Influence of pH on Enzyme Activities
How does the pH of solution influence the speed of enzyme activities?
Enzymes are protein catalysts and chemical that speed up chemical reaction without being involved in the reaction. They are involved in the human digestive system to help people digest food more efficient. Food are substrates to be break down and absorb through the digestive system in the body. Substrate is a molecule that bond with the active site of enzyme to transfer in to one or more products. It attaches to enzyme, form enzyme-substrate complex and leave enzyme unaffected (Eed). Most enzymes and substrate have their specific geometric shape, so Most enzymes act with only one substrate that …show more content…
The datas collected in this lab showed that both acid and basic solution slow down the enzyme activity and enzyme catalyse works better in neutral solution. The data overall shows that catalyses react slowest in acid solution and react fastest in the neutral solution. There is no reaction in ph 2 solution happened and the reaction in ph 4 solution is obviously slower than that in neutral solution and basic solution. In Graph #2, the highest point of reaction rate is at pH 7 and the lowest point of reaction rate is at pH 2. It shows that when pH is far from the optimum ph for enzyme activity, it will stop the activity. In pH 4 solution, the average time of reaction is 158 sec which is more than double of the average time of reaction in pH 7 solution. When pH is still lower but not that far from optimum pH, enzyme activity will obviously slow down. The time of reaction in basic solution is relatively faster than that in acid solution. The average time of reaction in pH 7 is only 82 sec and in pH 11.5 is 118 sec. Although it is still slower than the time of reaction in neutral solution, it indicates that catalyzes react better and faster in basic solution than acid solution. It also shows that pH solution that is higher than optimum pH for enzyme activity will slow down the enzyme activity. Graph #1 clearly indicates the curve of time change that support the hypothesis, If pH level of solution is higher or lower than seven, it will slow down or stop the rate of enzyme