What Are The Possible Factors Affect The Physicochemical Properties Of The Cryoprotectants?
During the data collection procedure, there were potential limitations that could possibly affect the validity of the data collected and the conclusions that could be drawn from my investigation.
The method optimisation study was based on trehalose, however, in an ideal world where no time restraints are present; the reconstitution study could be performed for each of the cryoprotectants to identify the most suitable method for each. This would then take into account the various differences that exist between the three cryoprotectants and thus their possible influences on reconstitution time and physicochemical properties of the CUR-CS-P-NPs.
Additionally, due to the DSC being booked up well in advance, being available only on specific days, …show more content…
The parameters used to determine the most suitable cryoprotectant were the preservation of nanoparticle size and surface charge, along with their morphology. From the method optimisation study, it was found that increasing the cryoprotectant: nanoparticle volume ratio to 1:1 without using the centrifugation process, proved to result in the simplest method of reconstitution, requiring only manual shaking to achieve full resuspension of the …show more content…
It has been suggested by N. Chung et al. [134] that a higher concentration of cryoprotectant results in a better nanoparticle re-dispersibility. Therefore, by finding the optimal concentration, the nanoparticles physicochemical properties pre-lyophilisation could be better maintained, thus ensuring the stability of the CUR-CS-P-NPs.
Furthermore, for the CUR-CS-P-NPs to be feasibly implemented as a potential treatment for colorectal cancer, they need to possess a long shelf-life, requiring them to be stable over a sustained period of time. Thus, a study that explores the efficacy of the various cryoprotectants on the long-term stability of the CUR-CS-P-NPs for up to 6-12 months should be carried out, in addition to determining the optimum storage conditions. This would then clarify which cryoprotectant offers the best protection for the nanoparticles under
The method optimisation study was based on trehalose, however, in an ideal world where no time restraints are present; the reconstitution study could be performed for each of the cryoprotectants to identify the most suitable method for each. This would then take into account the various differences that exist between the three cryoprotectants and thus their possible influences on reconstitution time and physicochemical properties of the CUR-CS-P-NPs.
Additionally, due to the DSC being booked up well in advance, being available only on specific days, …show more content…
The parameters used to determine the most suitable cryoprotectant were the preservation of nanoparticle size and surface charge, along with their morphology. From the method optimisation study, it was found that increasing the cryoprotectant: nanoparticle volume ratio to 1:1 without using the centrifugation process, proved to result in the simplest method of reconstitution, requiring only manual shaking to achieve full resuspension of the …show more content…
It has been suggested by N. Chung et al. [134] that a higher concentration of cryoprotectant results in a better nanoparticle re-dispersibility. Therefore, by finding the optimal concentration, the nanoparticles physicochemical properties pre-lyophilisation could be better maintained, thus ensuring the stability of the CUR-CS-P-NPs.
Furthermore, for the CUR-CS-P-NPs to be feasibly implemented as a potential treatment for colorectal cancer, they need to possess a long shelf-life, requiring them to be stable over a sustained period of time. Thus, a study that explores the efficacy of the various cryoprotectants on the long-term stability of the CUR-CS-P-NPs for up to 6-12 months should be carried out, in addition to determining the optimum storage conditions. This would then clarify which cryoprotectant offers the best protection for the nanoparticles under