Water diuresis in man

Topics: Urine, Sodium, Urination Pages: 10 (1290 words) Published: May 29, 2014
Water Diuresis in Man Practical Report
INTRODUCTION
The body needs to maintain equilibrium to function properly in everyday life. The most important substance it must regulate is water; water is everywhere in our body and its balance is essential for proper body function. A very carefully regulated process is solute concentration. If there is a sudden increase in water which enters the extracellular fluid, sodium ions will then contribute less to the extracellular solute concentration as the ratio between water and solute has now changed. Osmolality is the amount of solute in a kilogram, hence the osmolality in the extracellular space has also decreased. Water diuresis is the increase in urinary water excreted with little or no change in the solute excretion. Excess water needs to be excreted to maintain a good balance of water and solute inside the body. The aim of this practical is to test the effects of strenuous exercise and desmopressin (anti-diuretic hormone) on urine flow and urine sodium concentration. We will be testing the hypotheses that vigorous exercise will decrease urine flow and increase urine sodium concentration, whereas desmopressin will have the opposite effect of decreased urine flow and increased sodium concentration.

MATERIALS AND METHODS
Refer to the BMS2031 2014 Laboratory Manual.

RESULTS
Table 1. Details of the subjects in your experimental group. Subject
Sex (M/F)
Body weight
(kg)
Volume of water ingested (litres)
Procedure (control, desmopressin or exercise) 1
M
68
0.816
Exercise
2
M
120
1.44
ADH
3
F
62
0.744
Control

Table 2. Control Subject Data
Time at which bladder was emptied prior to practical class__-__

Sample 1
Drink water (12ml/kg body weight)
Sample 2
Sample 3
Sample 4
Sample 5
Sample 6
Time interval (min)
-

20
20
20
20
20
Volume (ml)
-

-
-
-
-
-
Urine flow (ml/min)
1.3

2.6
4.8
6.5
5.2
2.9
Na+ conc. (mmol/litre)
8.0

7.0
2.6
1.4
2.0
2.6
Na+ excretion rate (mol/min)
7.9

41.6
11.3
8.0
5.9
7.0

Note: Our control subject did not produce any urine, hence we used the class mean data so we would have some data to compare our other subjects to.

Table 3. Exercise Subject Data
Time at which bladder was emptied prior to practical class___9.00am___

Sample 1
Drink water (12ml/kg body weight)
Sample 2
Sample 3
Sample 4
Sample 5
Sample 6
Time interval (min)
300

20
20
20
20
20
Volume (ml)
190

50
110
160
80
10
Urine flow (ml/min)
0.6

1.7
5.5
8
4
0.5
Na+ conc. (mmol/litre)
5.83

7.27
2.42
1.11
2.63
25
Na+ excretion rate (mol/min)
3.6

11.9
13.31
8.88
10.52
12.5

Table 4. ADH Subject Data
Time at which bladder was emptied prior to practical class___12.56pm___

Sample 1
Drink water (12ml/kg body weight)
Sample 2
Sample 3
Sample 4
Sample 5
Sample 6
Time interval (min)
86

20
20
20
20
20
Volume (ml)
100

0
170
230
200
180
Urine flow (ml/min)
1.2

0
4.25
11.5
10
9
Na+ conc. (mmol/litre)
1.64

-
1.32
0.294
0.726
1.33
Na+ excretion rate (mol/min)
2.33

-
5.61
3.381
7.26
11.97

Figure 1. Effect of exercise and desmopressin on urine flow in group subjects

Figure 2. The effect of exercise and ADH on urine sodium concentration in group subjects

Figure 3. The effect of exercise and desmopressin on sodium excretion in group subjects

Figure 4. The effects of exercise and desmopressin on urine flow in class subjects

Figure 5. The effects of exercise and desmopressin on urine sodium concentration in class subjects

Figure 6. The effects of exercise and desmopressin on sodium excretion in class data

DISCUSSION (500 words)

Use the class data (refer to the appropriate figures in your discussion). (a) What happens to the rate of urine production (i.e. urine flow) for the three procedures...

References: Widmaier, EP, Raff, H & Strang, KT (2014). Vander’s Human Physiology. The Mechanisms of Body Function. MCGraw Hill, Chapter 14, page 499.
Continue Reading

Please join StudyMode to read the full document

You May Also Find These Documents Helpful

  • Essay about Water
  • Essay about The Man in the Water
  • Water and Electrolytes Essay
  • The Man in the Water Essay
  • idk man Essay
  • water Essay
  • water Essay
  • The Man in Water Essay

Become a StudyMode Member

Sign Up - It's Free