Urinary Chemistry Lab Report
The study of urinary chemistry on stone forming minerals will provide a good indication of risk of stone formation. Urinary supersaturation with respect to stone-forming constituents is generally considered to be one of the causative factors in calculogenesis. Though supersaturation of stone forming salts in urine is essential, abundance of these salts by itself will not always result in stone formation. Various substances in the body have an effect on one or more of the stone forming processes (nucleation, growth and aggregation and crystal retention), thereby influencing a person’s ability to promote or prevent stone formation.
Inhibitors of stone formation includes citrate, pyrophosphate, magnesium, and certain macromolecules like nephrocalcin, …show more content…
In liver the marker enzymes such as ALT and AST levels were recorded, whereas in kidney the levels of calcium, oxalate, phosphate, uric acid, creatinine, ALT and AST were conducted. The histopathological studies of kidney was also carried out.
The values obtained in all the biochemical analyses of urine, serum, liver and kidney were presented and discussed below.
For 24h urine collection, the animals were kept in metabolic cage and urine was collected on 0, 7th, 14th, 21st and 28th day of the study period. Animals had free access only to drinking water during urine collection period. The urine was analyzed for volume, pH, calcium, oxalate, phosphorus, uric acid, creatinine, magnesium and citrate.
Urolithiasis was induced by administration of ethylene glycol (0.75%) into healthy male Wistar rats. For in vivo analysis, 24h urine samples were collected on 0th, 7th, 14th, 21st and 28th day of treatment from all the rats of different treatment groups and the samples were preserved and stored. pH and the urine volume was recorded immediately after collection. The collected urine was used for further estimations for promoters of stone formation like calcium, oxalate, phosphorous, uric acid and creatinine. On 28th day the levels of inhibitors of stone formation namely magnesium and citrate were
Inhibitors of stone formation includes citrate, pyrophosphate, magnesium, and certain macromolecules like nephrocalcin, …show more content…
In liver the marker enzymes such as ALT and AST levels were recorded, whereas in kidney the levels of calcium, oxalate, phosphate, uric acid, creatinine, ALT and AST were conducted. The histopathological studies of kidney was also carried out.
The values obtained in all the biochemical analyses of urine, serum, liver and kidney were presented and discussed below.
For 24h urine collection, the animals were kept in metabolic cage and urine was collected on 0, 7th, 14th, 21st and 28th day of the study period. Animals had free access only to drinking water during urine collection period. The urine was analyzed for volume, pH, calcium, oxalate, phosphorus, uric acid, creatinine, magnesium and citrate.
Urolithiasis was induced by administration of ethylene glycol (0.75%) into healthy male Wistar rats. For in vivo analysis, 24h urine samples were collected on 0th, 7th, 14th, 21st and 28th day of treatment from all the rats of different treatment groups and the samples were preserved and stored. pH and the urine volume was recorded immediately after collection. The collected urine was used for further estimations for promoters of stone formation like calcium, oxalate, phosphorous, uric acid and creatinine. On 28th day the levels of inhibitors of stone formation namely magnesium and citrate were