Unknown DNA Restriction Enzymes

This experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab, it succeeds in showing the fragments. In this report we will discuss the, results, limitations and possible errors.
Introduction
In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown, with fragments of DNA from another source that the lengths are known. This process requires preparation of small fragments that are appropriate for nucleotide sequence analysis is to provide a map of the distribution of target sites. The cuts occur when palindromes show up in the DNA sequence; the different restriction endonucleases cut these different sites. This is called constructing a restriction map. In this experiment we are given an unknown DNA and use the process of DNA mapping with gel electrophoresis to compare and identify if the given DNA plasmid is of this planet and to describe what the DNA map illustrates.
Materials
Restriction endonucleases
Agarose
TBE buffer
Blue stain
These results were completed through the gel electrophoresis lab that was carried out. Gel electrophoresis is a lab technique used in biochemistry in order to separate out pieces of cut DNA. This process requires that you have an electrical field passing through the gel in a specific direction. To allow for charge to flow across, you must have something that will conduct electricity, a buffer that's usually composed of ions works as a conduit for the electrical charge. An agarose and TBE solution was prepared and used to completely cover the entire gel. The DNA has a negatively charged backbone because of all the phosphate groups so they moved towards the positive end of the gel