The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).
February 21, 2012
Staining and Identifying Unknown Bacteria
The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining, Capsule Staining, Endospore Staining, and Acid Fast staining. One of the most significant method of staining is the Gram Staining, as it is highly dependent (McCarthy, 25). In the specific experiment that was done, Gram Staining was used and the bacteria that was found was purple and round (cocci) shaped. Through this the bacteria was identified as Staphylococcus epidermis.
Material and Methods:
The first step to identifying the bacteria was to heat fix it to the slide. The materials used were a slide, water, a Bunsen Burner, bibulous paper and clothes pin. The unknown bacteria was in a vial in solid form. The steps on page 19 and 20 of the Customized Biol 311 General Microbiology Laboratory Manual were followed to heat fix the bacteria. After this gram staining was used to identify the unknown bacteria. The materials used for gram staining include the slide the was heat fixed, bibulous paper, crystal violet,