Transposon-Mediated Insertional Mutagenesis in Gene Discovery and Cancer
Transposon-mediated Insertional Mutagenesis in Gene Discovery and Cancer
This dissertation is submitted for the degree of Doctor of Philosophy By
Jun Kong
Team 113, Experimental Caner Genetics Group The Wellcome Trust Sanger Institute Wellcome Trust Genome Campus Hinxton, Cambridge CB10 1SA
Darwin College University of Cambridge Silver Street Cambridge CB3 9EU
DECLARATION
I hereby declare that this dissertation is the results of my own work and includes nothing which is the outcome of work done in collaboration, except the tumour watch and disease profiling studies for the Tel-AML1 mouse project in Chapter 4. This work was a collaboration with Dr. Louise van der Weyden, my colleague in the lab and Dr. Brian Huntly in MRCCIMR Cambridge. I have included some of their in vivo work to prove that this mouse model has been successful for modelling cALL (childhood Acute Lymphoblastic Leukemia) in human, and is included in Figure 4-9. None of the material presented herein has been submitted previously for the purpose of obtaining another degree. Material included in Chapter 2 has been published as Kong et al. (2008) Bioinformatics 24:2923-2925, material from Chapter 3 in Kong et al., (2010) Nucleic Acids Research 2010;38;18;e173 and Liang et al., (2009) Genesis 47:404-408. These publications are included as appendices. This dissertation does not exceed the word limit for the respective Degree Committee.
Jun Kong September 20, 2010
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ACKNOWLEDGEMENT
I would like to take this opportunity to extend my sincere thanks to all the people who generously supported me during my PhD study. I would like to first express my deepest thanks to my supervisor Dr. David Adams. Since the first time I met him in the Sanger institute, he has been given me so much help and support all through the years. While I was working with the mice from the beginning, he opened up my mind to explore other areas based on my interest and under his guidance most efforts afterwards
This dissertation is submitted for the degree of Doctor of Philosophy By
Jun Kong
Team 113, Experimental Caner Genetics Group The Wellcome Trust Sanger Institute Wellcome Trust Genome Campus Hinxton, Cambridge CB10 1SA
Darwin College University of Cambridge Silver Street Cambridge CB3 9EU
DECLARATION
I hereby declare that this dissertation is the results of my own work and includes nothing which is the outcome of work done in collaboration, except the tumour watch and disease profiling studies for the Tel-AML1 mouse project in Chapter 4. This work was a collaboration with Dr. Louise van der Weyden, my colleague in the lab and Dr. Brian Huntly in MRCCIMR Cambridge. I have included some of their in vivo work to prove that this mouse model has been successful for modelling cALL (childhood Acute Lymphoblastic Leukemia) in human, and is included in Figure 4-9. None of the material presented herein has been submitted previously for the purpose of obtaining another degree. Material included in Chapter 2 has been published as Kong et al. (2008) Bioinformatics 24:2923-2925, material from Chapter 3 in Kong et al., (2010) Nucleic Acids Research 2010;38;18;e173 and Liang et al., (2009) Genesis 47:404-408. These publications are included as appendices. This dissertation does not exceed the word limit for the respective Degree Committee.
Jun Kong September 20, 2010
Page | I
ACKNOWLEDGEMENT
I would like to take this opportunity to extend my sincere thanks to all the people who generously supported me during my PhD study. I would like to first express my deepest thanks to my supervisor Dr. David Adams. Since the first time I met him in the Sanger institute, he has been given me so much help and support all through the years. While I was working with the mice from the beginning, he opened up my mind to explore other areas based on my interest and under his guidance most efforts afterwards