Transformation Lab Summary

This lab was performed to see the Transformation abilities of, Escherichia coli into ampicillin resistant bacteria. For this lab, a total of six agar plates were used for growing the bacteria. Transformation occurred when plasmid DNA goes into the bacterial cell via a vector. The two ways change is encouraged is by setting them in calcium chloride (CaCl2) and applying the process of heat shock. These two strategies permitted the bacterial cells to end up capable or more open to the uptake of plasmid DNA. The reason for this analysis was to change bacterial cells with the plasmid containing ampicillin resistance and lux qualities. It was theorized that the

Lux qualities would have made a light-emitting response on the microbes while the ampicillin