Toxicology Lab

Throughout the week in the toxicology lab, I was acquainted with distinctive sort of tests and I got the opportunity to find out about the following:

1- hTSH test (Human thyroid stimulating Hormone):
- Add calibration and +/- controls, before starting the punching process.
- Add hTSH assay buffer + stock solution.
- Put the plate on a fast quick shaking for 10mins, and afterward 4 hours moderate shaking at room T°
-Add washing solution.
-Add enhancement solution, and read the result.
*The hTSH test is done when indications of hyperthyroidism are seen, and it is often requested along with a T4 test.
2- G6PD Test:
- The collected blood samples (on filter paper) are punched.
*Punching begins with the standards than 2 controls and finally
-Hydrolyze on the heat block for 25mins and then cool to room T°. - 0.5ml is added from the hydrolyzed solution, control and standard. -Add 20µl ammonia and vortex. - Complexing reagent is added (0.8ml) and vortex.

Cathecol: - 0.5ml is taken from sample, standard and control. - 50µl internal standard is added, then vortex. -13µl ammonia is added, then vortex. -Complexing reagent is added (0.8ml), then vortex.

Solid phase extraction (SPE) MET & Cat: -Take 1ml methanol. -1ml ammonia buffer (0.2M) is added. -The sample is added. -1ml of ammonia buffer + MeOH is added. -Dry using the centrifuge at high speed for 2 to 5 mins. -Change the disposable