Tisis
A Comparative Study of Dalandan and Kamias as Decolorizer in Gram’s Staining Staphylococcus aureus and Escherichia coli
An Undergraduate Research
Presented to
The College of Medical Technology
Centro Escolar University
In Partial Fulfillment
Of the Requirements of the Degree
Bachelor of Science in Medical Technology
By:
Aduana, Frances Mae D.
Barnachea, Erika Joy V.
Decleto, Pearl Yvette P.
Lagmay, Ira Alexis C.
Paris, Dublin Justin
Patron, Chronielle Albert C.
Radovan, Denzel James C.
Rodriguez, Angeline P.
Sale, Rwellein R.
ABSTRACT ABSTRACT The researchers conducted a study about Kamias (Averrhoa bilimbi) and Dalandan (Citrus aurantium) as alternative decolorizers in Gram Staining bacteria. Kamias (Averrhoa bilimbi) has a high oxalic acid content, fruit used to remove stains from clothing and for washing hands, removing rust and stains from metal blades. Dalandan (Citrus aurantium) is an aromatic variety of citrus that produces highly bitter, acidic fruits. Escherichia coli, a gram-negative bacillus that will appear as blue or violet when Gram stained, because it has a thick cell wall that prevents the primary dye (crystal violet) from being decolorized by alcohol. Staphylococcus aureus is a gram-negative coccus that will appear red when Gram stained because the peptidoglycan layer of its cell wall is thinner than that of Gram-positive bacteria, making the primary stain easier to decolorize.
ACKNOWLEGEMENTS
The researchers of this study would like to express their warmest gratitude to the following people for their kindness, support and guidance in order for the researchers to make their study possible: Ms. An Margaret Tan, Research Adviser, Centro Escolar University, for her guidance, patience in helping the researchers during their problems in their work and suggestions on how the research would be improved.
To the parents of the researchers for support and love that they give to the researchers
An Undergraduate Research
Presented to
The College of Medical Technology
Centro Escolar University
In Partial Fulfillment
Of the Requirements of the Degree
Bachelor of Science in Medical Technology
By:
Aduana, Frances Mae D.
Barnachea, Erika Joy V.
Decleto, Pearl Yvette P.
Lagmay, Ira Alexis C.
Paris, Dublin Justin
Patron, Chronielle Albert C.
Radovan, Denzel James C.
Rodriguez, Angeline P.
Sale, Rwellein R.
ABSTRACT ABSTRACT The researchers conducted a study about Kamias (Averrhoa bilimbi) and Dalandan (Citrus aurantium) as alternative decolorizers in Gram Staining bacteria. Kamias (Averrhoa bilimbi) has a high oxalic acid content, fruit used to remove stains from clothing and for washing hands, removing rust and stains from metal blades. Dalandan (Citrus aurantium) is an aromatic variety of citrus that produces highly bitter, acidic fruits. Escherichia coli, a gram-negative bacillus that will appear as blue or violet when Gram stained, because it has a thick cell wall that prevents the primary dye (crystal violet) from being decolorized by alcohol. Staphylococcus aureus is a gram-negative coccus that will appear red when Gram stained because the peptidoglycan layer of its cell wall is thinner than that of Gram-positive bacteria, making the primary stain easier to decolorize.
ACKNOWLEGEMENTS
The researchers of this study would like to express their warmest gratitude to the following people for their kindness, support and guidance in order for the researchers to make their study possible: Ms. An Margaret Tan, Research Adviser, Centro Escolar University, for her guidance, patience in helping the researchers during their problems in their work and suggestions on how the research would be improved.
To the parents of the researchers for support and love that they give to the researchers