The developing chamber is a critical technique of TLC for it becomes the principal reference to recording the Rf values of the solutions. 5ml of Ethyl Acetate Acid was poured inside a 250ml beaker. As stated in the introduction, CH3COOCH2CH3 was the developing solvent in our chamber for this solution. The beaker was then covered with a piece a foil and places under a hood. This was done so that the chamber would be ready as soon as the TLC plate prepared, as well as for security reason because of the level of toxicity of dichloromethane and it’s “possibility that prolonged inhalation […] may cause cancer.”1 (Lehman 145). For this reason, gloves and safety goggles were worn at all times. A TLC plate was obtained and with a pencil, 4 spots were made gently on its surface to serve as reference point to place the solutions. The points were respectively 1 cm from each other with the first and last point being at least 1.5 cm from their corresponding sides and all points were 1.5 cm from the bottom, to give time to the developing solvent to expand through all the points at the same rate once the TLC plate put in the developing chamber. Using capillary micropipettes, the solution were spotted to the
The developing chamber is a critical technique of TLC for it becomes the principal reference to recording the Rf values of the solutions. 5ml of Ethyl Acetate Acid was poured inside a 250ml beaker. As stated in the introduction, CH3COOCH2CH3 was the developing solvent in our chamber for this solution. The beaker was then covered with a piece a foil and places under a hood. This was done so that the chamber would be ready as soon as the TLC plate prepared, as well as for security reason because of the level of toxicity of dichloromethane and it’s “possibility that prolonged inhalation […] may cause cancer.”1 (Lehman 145). For this reason, gloves and safety goggles were worn at all times. A TLC plate was obtained and with a pencil, 4 spots were made gently on its surface to serve as reference point to place the solutions. The points were respectively 1 cm from each other with the first and last point being at least 1.5 cm from their corresponding sides and all points were 1.5 cm from the bottom, to give time to the developing solvent to expand through all the points at the same rate once the TLC plate put in the developing chamber. Using capillary micropipettes, the solution were spotted to the