Thesis ko sa AG
Laboratory Procedures in Serology
Collection and Preparation of Specimen
Fresh serum is always used
Blood-Collected in the morning, usually venipuncture
*Insert procedure here*
Heat Inactivation for Serum Specimen
*Insert procedure here*
Reagents are serological purposes are purchased as pre-packed kits. The antigen suspension is in a dropper bottle that delivers a requisive quality. The kit also contains buffer and other accessories required for performing the test. Saline (85% NaCl w/ deinonized water) is the only reagent prepared by the laboratory.
Serological tests are done in two ways
Slide method-Requires plate w/circles(wells) where the reagent in mixed Agglutination of flocculation is observed under a bright light. A rotator or rocker is used to mix the reagents
Tube method –Similar to slide method except that the reagent is placed in test tubes and is placed in a test tube rack.
Serological test for Syphilis
Syphilis is a sexually transmitted infection caused by the spirochete bacterium Treponema pallidum subspecies pallidum. The primary route of transmission is through sexual contact; it may also be transmitted from mother to fetus during pregnancy or at birth, resulting in congenital syphilis. Other human diseases caused by related Treponema pallidum include yaws
Nontreponemal tests refer to a class of syphilis diagnostics that detect infection by indirect markers of infection. Nontreponemal Tests detect biomarkers that are released during cellular damage that occurs from the syphilis spirochete. In contrast treponemal tests look for antibodies that are a direct result of the infection thus, anti-treponeme IgG, IgM and to a lesser degree IgA
Non Treponemal
Non-treponemal serologic tests for syphilis include VDRL (Venereal Disease Research Laboratory) test and RPR (Rapid Plasma Reagin) test. VDRL has been in use for several years now and the only difference between VDRL and RPR is that the latter does not need a microscope
Collection and Preparation of Specimen
Fresh serum is always used
Blood-Collected in the morning, usually venipuncture
*Insert procedure here*
Heat Inactivation for Serum Specimen
*Insert procedure here*
Reagents are serological purposes are purchased as pre-packed kits. The antigen suspension is in a dropper bottle that delivers a requisive quality. The kit also contains buffer and other accessories required for performing the test. Saline (85% NaCl w/ deinonized water) is the only reagent prepared by the laboratory.
Serological tests are done in two ways
Slide method-Requires plate w/circles(wells) where the reagent in mixed Agglutination of flocculation is observed under a bright light. A rotator or rocker is used to mix the reagents
Tube method –Similar to slide method except that the reagent is placed in test tubes and is placed in a test tube rack.
Serological test for Syphilis
Syphilis is a sexually transmitted infection caused by the spirochete bacterium Treponema pallidum subspecies pallidum. The primary route of transmission is through sexual contact; it may also be transmitted from mother to fetus during pregnancy or at birth, resulting in congenital syphilis. Other human diseases caused by related Treponema pallidum include yaws
Nontreponemal tests refer to a class of syphilis diagnostics that detect infection by indirect markers of infection. Nontreponemal Tests detect biomarkers that are released during cellular damage that occurs from the syphilis spirochete. In contrast treponemal tests look for antibodies that are a direct result of the infection thus, anti-treponeme IgG, IgM and to a lesser degree IgA
Non Treponemal
Non-treponemal serologic tests for syphilis include VDRL (Venereal Disease Research Laboratory) test and RPR (Rapid Plasma Reagin) test. VDRL has been in use for several years now and the only difference between VDRL and RPR is that the latter does not need a microscope