Roux, Daein, and Gaya, this summer I will help develop a way to split the BioID enzyme into two parts and then attach the parts onto two proteins of interest. When the two separate proteins then interact with each other, the BioID molecule will be complete and have the ability to biotinalate. Knowing that Jun and Fos interact with each other within the nuclease, we have attached parts of BioID to it. As of yesterday, we have confirmed that it is possible to split BioID and that it will still biotinalate, and now for the rest of my research I will be continuing to test different arrangements of BioID and BioID2. The testing will include repeated cloning, immunofluorescence, and western blotting. The purpose of cloning is to change the type of BioID inside of the cell, and the immunofluorescence and western blot will show us the
Roux, Daein, and Gaya, this summer I will help develop a way to split the BioID enzyme into two parts and then attach the parts onto two proteins of interest. When the two separate proteins then interact with each other, the BioID molecule will be complete and have the ability to biotinalate. Knowing that Jun and Fos interact with each other within the nuclease, we have attached parts of BioID to it. As of yesterday, we have confirmed that it is possible to split BioID and that it will still biotinalate, and now for the rest of my research I will be continuing to test different arrangements of BioID and BioID2. The testing will include repeated cloning, immunofluorescence, and western blotting. The purpose of cloning is to change the type of BioID inside of the cell, and the immunofluorescence and western blot will show us the