The Inverted Water Method Lab Report

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The inverted water method

MLA citation
BBC News. BBC, n.d. Web. 09 Nov. 2015.
"Factors Affecting Enzyme Activity." Factors Affecting Enzyme Activity. N.p., n.d. Web. 14 Nov. 2015.

Enzymes. Scenario experiment
AIM:
To investigate one factor that affect the distribution of hydrogen peroxide by the catalase in this organism. In this catalase and hydrogen peroxide experiment, we will find out how enzymes act as catalysts by causing chemical reactions to occur. Using a potato and hydrogen peroxide, we can observe how enzymes like catalase work to perform the breaking down, of other substances. We will look at enzymes and catalase and its substrate hydrogen peroxide. Catalase accelerates the breakdown of hydrogen peroxide (H2O2) into water
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Plot a graph of rate of oxygen production against concentration of hydrogen peroxide.
Pour the hydrogen peroxide into the test tube that contains the yeast and immediately put the gas syringe bung on the end of the test tube immediately , at the same time start the stopwatch. finish and record results and make a graph. accurate to help make this experiment more accurate i will repeat it 3 times and then use the average of all the results to plot a graph with a line of best fit. i tried keep all the variables except for the concentration of hydrogen peroxide the same for all the experiments. However, it is really hard to keep all the variables precisely the same. For example:
This will slightly affect all the results but as I carried out all the three steps in the same way for all the experiments it should not make any difference to the overall result. safety the way I will make sure I am safe when doing the experiment is I will wear eye protective goggles and protect my clothing from hydrogen peroxide since hydrogen peroxide is harmful and corrosive for the skin I will Report any accidents or spills. don't touch the hydrogen peroxide. Be aware of pressure building
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Catalase is an enzyme which breaks down hydrogen peroxide into water and oxygen

how does it work
Catalase works to speed up the decomposition of hydrogen peroxide into oxygen and water. since catalase is an enzyme it lowers the activation energy or how much energy is needed for a reaction to occur.

reaction of hydrogen peroxide
The foam produced in the reaction between hydrogen peroxide and potato is a result of catalase converting hydrogen peroxide into water and oxygen, the bubbles are filled with oxygen. The more bubbles produced the faster catalase is carrying out this reaction, or the more catalase present.

what is the effect of concentration against rate

Results

analysis of results when we increased the concentration of the hydrogen peroxide the rate of reaction increases at a relative even rate until the the concentration of Hydrogen Peroxide reaches about 4 to 5% When the concentration doubled from 3-6% the rate of reaction went up. 1.65 - 2.97 Cm3 Oxygen produced per second, which is an increase of 1.8. I expected the rate of reaction to increase twice when the Hydrogen Peroxide concentration was increased twice because there were twice as many substrate molecules which could join onto the active sites of the

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