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The Effects of Black Tea on the Growth of E. Coli Bacteria Cultures

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The Effects of Black Tea on the Growth of E. Coli Bacteria Cultures
The effects of black tea on the growth of E. coli bacteria cultures
Kierstin Barker, Melissa Bischak, Jackie Tyszkiewicz, Errin Enany
September 25, 2012
Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties, then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water, then the amount of bacteria exposed to this solution would decrease. We expected to see no difference in the amount of bacteria exposed to the black tea solution. Tannic acid, black tea, boiled water, and a tetracycline antibiotic were tested for their zones of inhibition in order to determine antimicrobial properties. Our results stated that tea does not contain tannic acid but does contain tannin and therefore does not have antimicrobial properties that contain tannic acid based on the results of this experiment.
Introduction:
In the novel: The Ghost Map, written by Steven Johnson, Johnson refers to the idea that tea has antimicrobial properties and that it could possibly have influenced people’s risk of contracting infectious diseases such as cholera (Johnson, 2006). Traditionally, people drank tea to improve blood flow, eliminate toxins, and to improve resistance to diseases (Dufresne and Farnworth, 2000). Tea has been shown to have a wide range of physiological and pharmacological effects such as having antimicrobial properties, inhibiting malignant cells, and simply leaving the drinker feeling refreshed (Hamilton-Miller, 1995). Black tea contains amounts of tannic acid. Tannic acid is a commercial type of tannin, which is a bitter plant polyphenolic compound that binds to and precipitates proteins and various other organic compounds including amino acids and alkaloids and has antimicrobial properties which could possibly aid in warding off waterborne diseases (Yildirim, 2000).
Could some



Cited: Dufresne, C. and Farnworth, E. 2000. A review of latest research findings on the health promotion properties of tea Hamilton-Miller, J.M.T. 1995. Antimicrobial Agents and Chemotherapy, Vol. 39, No. 11. London: American Society for Microbiology. Johnson, S. 2006. The Ghost Map. New York: Riverhead Books. Lee Anne A. Drug Information Online, 2012. “Tetracycline Hydrochloride Capsules” USPhttp://www.drugs.com/pro/tetracycline.html Lee Anne Anderson (September 20, 2012). McGee, H. Tea 101, 2006. “What are Tannins?” http://www.ochef.com/197.htm (September 21, 2012). Post, Gordon. Post Apple Scientific Inc, 2009. “Tannic Acid” http://tannicacid.info/ (September 21, 2012). Richardson, B. 2012. Tannic Acid in Tea? I Don’t Think So. The Serene Cup. Tiwari TP, Bharti SK, Kaur HD, Dikshit RP, Hoondal GS. 2005. Synergistic antimicrobial activity of tea & antibiotics Yildirim, A. 2000. J. Agric. Food Chem., Vol. 38, No. 10. Turkey: Erzurum.

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