The Effect of Enzyme Concentration on Rate of Reaction
The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills, the experiment was done in order to determine how the rate of reaction will be altered, whether it will increase, decrease or remain constant when the different concentration of enzymes added.
Enzymes are produced naturally in plant, animal, and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules that are greatly increase the rate of chemical reaction without themselves being changed at the end of the reaction. A chemical reaction involves the conversion of a substrate into a product. This reaction is normally known as an enzyme-catalyzed reaction. In this reaction, the substrate will bind to the active site of the enzyme. Once it is bind, an enzyme-substrate complex is formed. At this form, the substrate is converted into product. Finally, the product will be released.
There are four factors that affect the enzyme activity that is temperature, pH, substrate concentration and enzyme concentration. As for temperature, it will affect the enzyme activity when there is a rise in temperature. When temperature increases, this will automatically increase the kinetic energy. This will then results in the increase of the rate of collision between the enzyme and substrate, but this effect is limited. The rate of reaction will increase up to a certain temperature. At this temperature, the enzyme activity is the greatest. Changes in pH will results in disruption of hydrogen bond of the protein chain. Hence, this will results in changes in shape of the enzymes' active site that then will reduce the effectiveness of the enzyme and the rate of reaction.
Using different type of substrate concentration will also affect the rate of reaction. Increase in substrate concentration will increase the rate