Biology For Majors
October 4, 2012
We examined the reaction an enzyme has when its concentration, pH and temperature are altered. In order to do this, we added different levels of pH into different test tubes with the enzyme (sucrose), and substrate (sucrose), and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature increases. Oppositely, low temperature levels resulted in little to no change or the enzyme denatured. Temperature variations were observed and recorded. Substrate concentration testing showed that as when the substrate increased, the activity of the enzyme also increased.
Keyword: enzyme, substrate, sucrose, sucrose
The Effect of Concentration, pH and Temperature on Enzyme Activity Introduction
In this lab we explore enzyme activity and how it can be affected by changes to its environment. Enzymes are proteins that speed up the rate of chemical reactions. Many important processes in the body involve the work of enzymes, including the digestion of nutrients such as carbohydrates, proteins and fats (Raven 45). Enzymes are also organic catalysts. A catalyst is a chemical that controls the rate of a reaction, but is itself not used up in the process. Reactions that are accelerated due to the presence of enzymes are known as enzyme-catalyzed reactions (Raven 112). The substrate is the reactant within the reaction that fits with the enzyme. Once the substrate enters the enzyme’s active site, the enzyme’s shape changes to form an enzyme-substrate complex. The substrate is then metabolized or broken down, resulting in a product, which can be utilized to energize cells. Once the product is released from the active site, the enzyme returns to its original shape (Raven 117). The three factors that can affect the activity of an enzyme include