The materials used in this experiment were a scale, 24" of dialysis tubing, 4 transfer pipettes, sugar, scissors, rubber bands, four coffee cups, a 250ml graduated cylinder, a ruler, a small sauce pan, and 3 clean containers.…
When a substance is released into an area, the random movement of its molecules results in a multitude of collisions. These collisions, in turn, lead to a dispersion of the molecules. The overall movement of the molecules will be from an area of high concentration, where there will be more collisions, to areas of low concentration, where the number of collisions will be much less. This process of dispersion will continue until there is no net gain or loss of molecules in an area. The process by which this equilibrium occurs is called diffusion. Diffusion is vitally important to biology on many levels; individual cells, organelles, and even whole organisms rely on diffusion to carry out the processes essential to life. One especially important aspect of diffusion is osmosis, or the diffusion of water. This often occurs across a semi-permeable membrane…
Fill 4 dialysis tubes 2/3 of the way with 1 M glucose solution. Fill 4 beakers with 100 mL of 1 M sucrose. Weigh all four bags before placing them into beakers. Record temperature of room temperature sucrose and place one bag into that beaker for ten minutes. After ten minutes, take the bag out, dry, and weigh. Heat up two beakers of sucrose solution to two different temperatures, record the temperatures, and place the bags in for ten minutes. After ten minutes, take the bags out, dry, and weigh. Cool down one beaker full of sucrose with ice, record the temperature, and place the last bag into the beaker. After ten minutes, take the bag out, dry, and weigh.…
1. The cell membrane regulates and controls what kind of molecules ______ move in & out of the cell.…
Seal the end if it is not already closed. Next, pick up a pipette and fill it with glucose. (Use this pipette only for glucose) then release the glucose into the dialysis tubing. Place four more pipettes full of glucose into the dialysis tubing. Now it is time to get another pipette (not the one used for glucose) and fill it with starch and release it into the dialysis tubing. Repeat this 4 (four) more times until there is a total of 5 pipettes of glucose and 5 pipettes of starch in the dialysis tubing. Mix the solution. Next, seal the dialysis tubing completely and make sure there is little to no air left in the dialysis tubing. Rise the bag with water. Next, fill a 400ml beaker with 300ml of water. Add 5 pipettes of Lugol´s into the 300ml of water. Mix the solution gently. Place the dialysis tubing bag into the 400ml beaker and leave for 30 minutes. After 30 minutes remove the bag and record your findings. Get 3 test tubes and label them “control” “bag” and “beaker” Next fill an unused pipette with water and pour it into the control test tube. Open the dialysis tubing and fill a pipette of the solution inside using an unused pipette. Repeat again and pour into the bag test tube. Place two pipettes of the beaker solution into the beaker tube. Add one pipettes of Benedict´s into each tube Heat all tubes for three minutes. Record…
We tied one end of the glucose tubing, and put 2 inches of glucose solution into it then we tied the other side into a knot to close it up. With the second dialysis tube we did the same thing but with the starch solution. We then weighed the glucose cell, then the starch cell, and recorded the mass into the chart. After that we placed each cell into a seperate cup, and filled it with water. We let the cells sit in the cups overnight. The next day we got our cup with the glucose cell, we took a glucose test strip and dipped it into the water in the cup to test for glucose. Then we took the cell out of the water, weighed it, and recorded it in the chart. We took the cup with the starch cell and tested it for starch by using lugols iodine. We put 4 drops of the iodine into the cup. We took the starch cell and weighed it, then recorded…
Diffusion and Osmosis are two concepts that go hand in hand with each other. Diffusion is simply described as the movement of a substance from a region of high concentration to a region of low concentration. In another words, the substance will move down its concentration gradient which is “the region along which the density of a chemical substance increases or decreases” (Campbell Biology pg. 132). If you understand the concept of diffusion then osmosis is a very simple process. It can be defined as the diffusion of water across a permeable membrane. Osmosis can be cellular or artificial, so even though we are creating artificial cells in this lab, it is still considered to be osmosis. During osmosis, a solvent is trying to get through a selectively permeable membrane to make the concentration of that solvent the same on both sides of the membrane. The rate of osmosis depends on the type of environment the cell is in. There are three different environments that a cell can find itself in, a hypertonic environment, a hypotonic environment, or an isotonic environment. The environment a cell is in will determine its tonicity which is “the ability of a surrounding solution to cause a cell to gain or lose…
The experiment begins by softening up the dialysis tubes by soaking them in a beaker of water. When softened, rub the dialysis tubes between your fingers to reveal the opening and clamp the opposite end. Add 10 mL of the appropriate solution to each bag, squeezing the air out of the tube to make sure there are no air bubbles when clamping the other end of the tube. Three of these bags will be filled with 20%, 40% and 60% sucrose solutions. The other two will contain water. Weigh each bag on a balance after clamping and record initial weight. Fill each beaker with the appropriate solution, just enough to cover the bag; four will have water and the fifth beaker will hold 40% sucrose. The bags containing 20%, 40% and 60% will be placed in water, as well as one bag filled with water; the bag of water in water will act as your control. The other bag of water will be placed in the beaker containing 40% sucrose solution. Place the five bags in their rightful beaker simultaneously and record the time. Every ten minutes the bags should be removed, blotted to get the excess solution off and then weighed. Continue weighing the bags every ten minutes for ninety…
Exercise 1B: First, soak six 30-cm of dialysis tubing in water. Then, take each one out and tie a knot on one end of each piece to make a bag. Pour 25 mL of a different solution (distilled water, 0.2 M sucrose, 0.4 M sucrose, 0.6 M sucrose, 0.8 M sucrose, and 1.0 M sucrose) into separate bags. Tie the other end of the bag, leaving sufficient space for expansion. Record the initial mass for each bag in grams. Then, fill the cups with distilled water. Place each bag in one cup and label each cup with each solution. After 30 minutes, remove the bags from the water. Finally, record each bag…
The dialysis tube was rinsed with tap water and the contents were emptied into a 100mL beaker.…
4. Using a pipette, all three tubes were filled with approximately 5 mL of 5% sucrose solution.…
Osmosis is the diffusion of a solvent across a selectively permeable membrane that occurs in response to differences in solute concentrations (Allen and Harper 2014).Osmosis can fall under the category of passive transport which does not require energy. With osmosis being a type of diffusion it is viewed as molecules moving from a high concentration to a low concentration. To further explain if there is a low water concentration, high amounts of solutes will be present. Water will most likely move to areas where the solute concentration is high, which demonstrates why there would be less water concentration.…
In this I will be investigating what effects the movement of water through osmosis. Osmosis is the diffusion of water. It is the process in which fluids pass through a partially-permeable membrane. It is the movement of water from high water concentration to low water concentration. Plant cells react to osmosis by hypertonic, isotonic and hypotonic.…
During the past few weeks in my Ag Biology class we have been learning about and reviewing both osmosis and diffusion. We did two different labs, one on osmosis; the other on diffusion.…
The materials that were used to set up the experiment were 4 bags of dialysis tubing, 8 clamps, and 4 beakers. The 4 bags will be filled with different solutions one being tap water, the…