the effect of catalase on hydrogen peroxide

Topics: Enzyme, Enzyme inhibitor, Catalysis Pages: 7 (1225 words) Published: March 23, 2014
THE EFFECT OF CATALASE ON
HYDROGEN PEROXIDE

The aim of the experiments is to see if increasing the surface area of the enzyme Catalase, affects the relative activity of the substrate Hydrogen peroxide. Then to observe and measure the effect the Catalase has on the chemical breakdown of the hydrogen peroxide.

My theory is if you keep increasing the surface area of Catalase, the more active sites are available to join with the substrate causing an increase in the breakdown of the hydrogen peroxide producing more oxygen and water. There will be an optimal point when all the active sites are filled, at this point the reaction will stop, as all the hydrogen peroxide will have been broken down leaving only Catalase, water and oxygen.

I predict the more you increase the surface area of Catalase, the more oxygen produced, because the hydrogen peroxide has more surface area to collide with, causing a faster rate of reaction.

Apparatus/equipment needed
Size 6 hole borer
A tile
2 x measuring cylinders
Conical flask
Bung + delivery tube
Knife
Potatoes
Stop watch
Hydrogen peroxide
Boss + clamp + stand
Water
Plastic container

Method
1. Put on safety glasses
2. Set up equipment as shown above
3. Use the hole borer and bore out pieces of potato then cut into 2cm lengths. 4. Put 1x2cm piece of potato in the conical flask with 50ml of hydrogen peroxide. Replace bung immediately and start stopwatch for 5 minutes. 5. After 5 minutes pull out bung, read the measurement on the upside down measuring cylinder. Record your results 6. Empty and rinse conical flask.

7. Refill upside down measuring cylinder with water
8. Repeat steps 3-7 another 4 times but in step 4 add another 2cm piece of potato each time. 9. Perform the experiment three times
10. Take the room temperature at the start middle and end of the experiment 11. Repeat the steps 3-7 this time omit the potato, this gives the control result. You only need do this once.

RESULTS

Oxygen levels
SURFACE AREA OF POTATOE
Control 0
1X2CM
7.39cm³
2X2CM
14.78cm³

3X2CM
22.17cm³
4X2CM
29.56cm³
5X2CM
36.95cm³

1ST results
4ml
4ml
9ml
10ml
13ml
2ND results
4ml
6ml
8ml
10ml
12ml
3RD results
3ml
5ml
9ml
12ml
14ml

I collected extra results from 2 class members.
Oxygen levels
SURFACE AREA OF POTATOE
Control 0
1X2CM
2X2CM
3X2CM
4X2CM
5X2CM

1ST results
0.5ml
7ml
9ml
15ml
18ml
2ND results
3ml
5ml
12ml
17ml
21ml
3RD results
3ml
8ml
9ml
14ml
23ml
1ST results
4ml
5ml
7ml
8ml
18ml
2ND results
2ml
7ml
8ml
10ml
11ml
3RD results
3ml
6ml
7ml
9ml
14ml

Reading
Room temperature
1
23˚c
2
23˚c
3
23˚c

A table showing combined averages

Surface area of potato
Average levels of oxygen
1x2cm
3
2x2cm
4.7
3x2cm
8.7
4x2cm
11.7
5x2cm
16

Enzymes are complex chemicals that control chemical reactions in the body. Enzymes are biological catalysts that speed up chemical reactions, without being used up or changed. They do this by reducing the amount of activation energy that’s needed, making reactions happen at a lower temperature. Enzymes are effective in the body, because they lower the amount of energy required for a reaction to happen

All enzymes have an active site.(Johnson 2013) This is where another molecule can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme, it converts the substrate into one or more products. Enzymes are specific to their substrate, because the shape of their active site will only fit the shape of their substrate. It is said that the enzymes active site is complimentary to their substrate. This is known as the lock and key theory. If the substrate’s shape doesn’t match the active site shape, then reaction won’t be catalysed.

Another theory is the Induced fit theory it is when the substrate and enzyme fit together but the enzyme-substrate complex changes shape a little to...


References: Johnson.M.D(2013)Human biololgy:concepts and current issues 7th edition, Benjamin cummings publishing
Bowness.E.(2009)A2-LEVELBiology:coordination group publications LTD
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