Synthesis and Antibacterial Activity of Silver Nanoparticles with Different Sizes

J Nanopart Res (2008) 10:1343–1348 DOI 10.1007/s11051-008-9428-6

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Synthesis and antibacterial activity of silver nanoparticles with different sizes
´ ´ ˜´ ˜ G. A. Martınez-Castanon Æ N. Nino-Martınez Æ ´ ´ F. Martınez-Gutierrez Æ J. R. Martınez-Mendoza Æ Facundo Ruiz

Received: 29 January 2008 / Accepted: 22 May 2008 / Published online: 2 July 2008 Ó Springer Science+Business Media B.V. 2008

Abstract Silver nanoparticles with different sizes (7, 29, and 89 nm mean values) were synthesized using gallic acid in an aqueous chemical reduction method. The nanoparticles were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), and ultraviolet–visible (UV–Vis) absorption spectroscopy; the antibacterial activity was assessed using the standard microdilution method, determining the minimum inhibitory concentration (MIC) according to the National Committee for Clinical Laboratory Standards. From the microscopies studies (TEM) we observed that silver nanoparticles have spherical (7 and 29 nm) and

pseudospherical shape (89 nm) with a narrow size distribution. The sizes of the silver nanoparticles were controlled by varying some experimental conditions. It was found that the antibacterial activity of the nanoparticles varies when their size diminishes. Keywords Antibacterial-activity Á Ag-nanoparticles Á Synthesis Á Nanobiotechnology Á EHS

´ ˜´ G. A. Martınez-Castanon (&) ´ Maestria en Ciencias Odontologicas, Facultad de ´ Estomatologıa, UASLP, Av. Manuel Nava 2, Zona Universitaria, San Luis Potosi, SLP, Mexico e-mail: [e-mail] ´ ˜ N. Nino-Martınez Instituto de Metalurgia, UASLP, Av. Sierra Leona ´ No. 550, Col. Lomas 2a. Seccion, San Luis Potosi, SLP, Mexico ´ ´ ˜ N. Nino-Martınez Á J. R. Martınez-Mendoza Á F. Ruiz ´ ´ Facultad de Ciencias, UASLP, Alvaro Obregon 64, C.P. 78000 San Luis Potosi, SLP, Mexico ´ F. Martınez-Gutierrez ´ Facultad de Ciencias

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