Preview

Syneresis Lab Report

Good Essays
Open Document
Open Document
764 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Syneresis Lab Report
There are several methods to test syneresis. Acid curds need to be formed in a glass beaker which make it easier to transfer the curds into syneresis test installations. All curds must be transferred carefully into the device, otherwise some soft curds were easily broken and the results would be affected. In case that some of the gel were not easily removed with the sample residues on the bottom of the beaker when transforming, beakers can be pre-coated with paraffin wax on the inside. Both a barrel-shaped filter and a semicircle filter can be used in this experiment (Fig. 1: a, b), but the test results were more precise when using semicircular filters (data not shown). The reason might be the pressure that the barrel-shaped filter fold give to the curd was uneven. Compared to the centrifugation and cutting method mentioned in the …show more content…
Rather, it is likely to be the result of a range of chemical interaction that are enabled by and developed on physical agitation of the curd. Renault et al. (1997) found that syneresis could be influenced by changes in the protein conformation, essentially due to hydrophobic bounding. Many authors (Pearse et al. 1985; Stoll 1966; Walstra et al. 1985) indicated that whey protein denaturation syneresis rate can influence syneresis rate. Denatured whey protein rate is increased with increasing heat treatment temperature. The observed differences in syneresis can be related to the differences in curds structure that were demonstrated by SEM (Fig. 3). Syneresis rate and the kinetic rate constant k of curds were higher for the gels having larger pores, which can be inferred by the syneresis changes (Table 2 ) and microstructure changes (Fig. 3B) of rennet curds made from different pH and temperature. Similar results had been reported by Pierre-Anton et al. (2003). The results also showed that lower pH acid curds had larger pores and denser

You May Also Find These Documents Helpful

  • Good Essays

    Erlenmeyer Synthesis

    • 998 Words
    • 4 Pages

    In the second separation of this experiment, the Erlenmeyer flask with the first filtrate was put into a beaker so the filtrate was in the beaker. The beaker was then heated on a hotplate until near boiling. After the whey protein had precipitated, a vacuum filter was used to filter out the whey. These proteins were then air-dried. A qualitative test was done to indicate that the sample extracted was the protein whey. Again, a small test tube was used and 2 mL of protein test solution was put in the tube. Five drops of 10% NaOH and 2 drops of 0.5% CuSO4 were added to this tube. If a purple color showed, it would indicate there was protein in the test tube.…

    • 998 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Anti-Cow Serum Lab Report

    • 1052 Words
    • 5 Pages

    The stacking gel was used to make sure all the proteins start separating at about the same time. It has a larger pore size so that the larger protein can move in easily. The SDS and heating was used to denature the proteins, the proteins then loaded into the lane. When apply the electrical field, the proteins will move toward the bottom, which is the positive anode. Also, the Tris-glycine will enter the stacking gel. The pH of stacking gel is 6.8, it makes the proteins less negative because the amino acids are protonated at equilibrium. Because the glycine moves slower than chloride ion, a Gly-chloride ion boundary is formed. But, glycine still runs little bit faster than other proteins. Therefore, the proteins are trapped between chloride ion and glycine. A very tight band is formed inside the stacking gel by the…

    • 1052 Words
    • 5 Pages
    Better Essays
  • Satisfactory Essays

    Quiz010: Lab Report

    • 929 Words
    • 4 Pages

    | LabRepQuiz010 Question MC #7: Which of the following is the best written sentence that includes the information below taken from an article written by Dr. Costanza, but avoids plagiarism?…

    • 929 Words
    • 4 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Nerve Impulses Lab Report

    • 647 Words
    • 3 Pages

    Name:shasou Exercise 3: Neurophysiology of Nerve Impulses: Activity 5: The Action Potential: Measuring Its Absolute and Relative Refractory Periods Lab Report Pre-lab Quiz Results You scored 50% by answering 2 out of 4 questions correctly. 1. Which of the following occurs after the peak of the action potential? Your answer : b. Voltage-gated K+ channels open. Correct answer: d. All of these occur. 2. What is meant by Na+ channel inactivation? Your answer : a. The Na+ channel opens when the membrane reaches threshold. Correct answer: b. The Na+ channel no longer allows Na+ ions to pass through it. 3. What happens when voltage-gated K+ channels open? You correctly answered: d. All of these occur. 4. It is harder to generate a second action potential…

    • 647 Words
    • 3 Pages
    Satisfactory Essays
  • Better Essays

    Lab Report Part II

    • 1247 Words
    • 4 Pages

    Purpose: To be familiarized with the science and techniques used to identify different types of bacteria based on their DNA sequences.…

    • 1247 Words
    • 4 Pages
    Better Essays
  • Good Essays

    lab report 1

    • 589 Words
    • 3 Pages

    Purpose: to test cohesion and adhesion of distilled H2O, tap H2O, salt H2O, and soapy H2O…

    • 589 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Lab Report

    • 597 Words
    • 3 Pages

    The purpose of this experiment is to see whether or not temperature plays a role in the percent change during diffusion or osmosis.…

    • 597 Words
    • 3 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Lab Report

    • 317 Words
    • 2 Pages

    In this lab, we will take a trip to the planetarium lab and will learn about Right ascension and declination, and altitude and zenith. After looking at the different points shown, we will log the altitude and zenith in the chart in our lab manual. Now we will look at the same points and label the right ascension and declination. Then we will learn about the easiest way to locate the star Polaris.…

    • 317 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Lab Report 1

    • 1241 Words
    • 5 Pages

    Purpose: In this lab, I will observe macroscopic changes that occur when mixing together certain chemicals and household cleaners. The mixing of the chemicals provided with each other and with acid/base indicators demonstrates how matter can change, and how chemistry can be seen with the naked eye. By mixing household cleaners with an acid/base indicator I hope to demonstrate how these changes can be related to everyday life.…

    • 1241 Words
    • 5 Pages
    Good Essays
  • Good Essays

    Lab Report

    • 366 Words
    • 2 Pages

    First, I would take a sample from different areas of the water to test the amount of dissolved oxygen in the water. Then after completing this test, I would check to see if there is” of course “an increase in the fish present in the water. This observation would help keep track of the fish present in different areas of the water and furthermore, I would be able to compare results.…

    • 366 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Lab Report

    • 623 Words
    • 3 Pages

    The number of electrons forming a charge cloud around the nucleus is (pick one of the following) greater than; equal to; smaller than the number of protons in the nucleus of the atom.…

    • 623 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Lab Report

    • 738 Words
    • 3 Pages

    3 Watch each part of the experimental demonstration and make preditions about wht will happen in each scenario . Record your preditions and observations in the Data an Observations section of your labatory report format.…

    • 738 Words
    • 3 Pages
    Satisfactory Essays
  • Powerful Essays

    Lab Report

    • 1455 Words
    • 6 Pages

    Use the results of the reaction to determine the mass and moles of Cu and Cl in the reaction.…

    • 1455 Words
    • 6 Pages
    Powerful Essays
  • Good Essays

    In our hypothesis we predicted that the enzymes in the pineapple will denature when the temperature is changed. The prediction was partly correct. Pineapples have special enzymes that denature gelatin molecules. Which is the reason why the pineapple and gelatin test tube remained filled with liquid, instead of turning into jelly which gelatin does when it’s molecules are not disturbed. Why our hypothesis was partly incorrect was lacked depth in that when changing the temperature by heating the pineapple to 40C it actually increased the speed and efficiency of the reaction taking place and increasing the amount of gelatin broken down by the enzymes in the pineapple, because increasing the heat also increases the processes of an enzyme. But once it hit a temperature high enough to completely alter the pineapple enzymes the results showed what we had predicted, the gelatin hardened because the pineapple's hydrogen bonds, that hold the enzyme together, broke and the enzyme unfolded, disrupting the overall structure of the protein. The substrate could no longer bind to the enzyme's active site, and the enzyme could not catalyze the reaction. Fresh Raw Pineapple enzymes actively broke down the gelatins molecules throughout all of its trials. When it was heated to 40C it increased the reaction and helped break down the molecules, and at 75C some of the enzymes in the pineapple began to denature but most of the enzymes remained intact and continued to break down the gelatin because the temperature was not high enough or the time heated was not long enough to denature. The Fresh Boiled Pineapple began at .03 percent because of the fact it was recently boiled and the enzymes were almost all completely denatured, but after cooling and then being heated to only 40C the reaction was sped up and the reaction of breaking down gelatin continued. But once the freshly boiled pineapple was heated to 70C all the enzymes that were left all became completely denatured, leaving 0 liquid…

    • 623 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Lab report

    • 708 Words
    • 3 Pages

    The overall goal of experiment four was to determine the identity of unknown cations presented to the student. But in order to know the identity of these unknowns, in part 1, Ag+, Pb+, and Hg22+ were presented to the student in aqueous solutions and then precipitated through experimentation. In part 2, the same procedure was enacted to determine which substances precipitated through qualitative analysis. Solubility rules were also a major theme as solubility is important in determining whether a reaction will produce a precipitate.…

    • 708 Words
    • 3 Pages
    Good Essays