Sugar Accumulation in Grape Berries

Topics: Fruit, Tomato, Grape Pages: 9 (7304 words) Published: October 30, 2014
Plant Physiol. (1 996) 1 1 1 : 275-283

Sugar Accumulation in Grape Berries
Cloning of Two Putative Vacuolar lnvertase cDNAs and Their Expression in Grapevine Tissues
Christopher Davies* and Simon

P. Robinson

Cooperative Research Centre for Viticulture, P.O. Box 145, Glen Osmond, South Australia, Australia, and Commonwealth Scientific and industrial Research Organization, Division of Horticulture, G.P.O. Box 350, Adelaide, South Australia 5001, Australia

The growth pattern of developing grape berries (Vifis
vinifera L.) can be described as a double sigmoidal curve
with an initial rapid increase in size followed by a lag
period during which berry volume does not increase. The
lag period is followed by a second phase of growth during
which ripening occurs, and viticulturists use the French
word véraison to describe the inception of berry ripening.
During ripening, the increase in volume is accompanied by
an increase in berry softness, accumulation of hexoses in
the berries, and a decrease in the leve1 of malic and tartaric acids, and in red grape varieties the skin becomes colored
due to the accumulation of anthocyanins (Coombe, 1992).
The accumulation of sugar in the form of Glc and Fru
within the vacuole is one of the main features of the ripening process in grape berries and is a major commercial consideration for the grape grower, winemaker, and dried
fruit producer. Sugar accumulation in climacteric fruit has
received considerable attention, but little is known about

this process in nonclimacteric fruit such as grapes. In
grapevines, SUCproduced as a result of photosynthesis in
the leaf is transported via the phloem to the berry (Swanson and Elshishiny, 1958), where it is cleaved to Glc and Fru, which accumulate in roughly equal amounts (Kliewer,
1965).The accumulation of Fru and Glc commences only at
véraison and continues throughout ripening.
Invertase (P-fructosidase; EC catalyzes the conversion of Suc to its monosaccharide constituents Glc and Fru and is probably responsible for this reaction in grape
berries as ít is in other species that store sugar in this way. In tomato, species that accumulate high levels of Glc and
Fru, for example Lycopersicon esculentum Mill., have high
levels of invertase activity and store little Suc (Yelle et al., 1991; Stommel, 1992). In contrast, accumulators of high
levels of Suc (and low levels of Glc and Fru), such as
Lycopersicon peruvianum and Lycopersicon chmielewskii, have
low levels of vacuolar invertase. Further evidence for a role for vacuolar invertase in controlling the composition of
sugars in tomato fruit has recently been provided by antisense (Ohyama et al., 1995) and breeding experiments (Chetelat et al., 1995).
A number of distinct invertases have been characterized
in plants. Most reports deal with either soluble invertases, which have acidic pI values and acid pH optima and are
located in the vacuole, or with insoluble acid invertases,
which have basic pI values and are bound to the cell wall
(Sturm and Chrispeels, 1990). It is thought that the vacuolar invertases are likely to be important in the regulation of hexose levels in certain tissues, e.g. in fruit tissue (Lingle and Dunlop, 1987), and in the utilization of Suc stored in

the vacuoles (Leigh et al., 1979). The cell-wall forms are
associated with rapidly growing tissues (Eschrich, 1980),
are induced by wounding and pathogenic attack (Sturm
and Chrispeels, 1990), and have been implicated in phloem
unloading and source / sink regulation (Eschrich, 1980;
Roitsch et al., 1995).
Hawker (1969a) found that invertase enzyme activity in
Sultana berries increased immediately after flowering and
that the activity peaked 6 to 7 weeks later, at véraison, when

* Corresponding author; e-mail; fax 618-303-8601.

Abbreviations: "Brix, refractive index measure of total soluble solids; RACE, rapid amplification of cDNA ends....

Cited: Arai M, Mori H, Imaseki H (1992) Cloning and sequence for an
intracellular acid invertase from etiolated hypocotyls of mung
Plant Cell Physiol33: 245-252
Chetelat RT, DeVerna JW, Bennett AB (1995) Effects of the Lycopersicon chmielewskii sucrose accumulator gene (sucr) on fruit
yield and quality parameters following introgression into tomato. Theor Appl Genet 91: 334-339
Coombe BG (1992) Research on development and ripening of the
grape berry. Am J Enol Vitic 43: 101-110
Coombe BG, Bishop GR (1980) Development of the grape berry.
Aust J Agric Res 31: 499-509
Dali N, Michaud D, Yelle S (1992) Evidence for the involvement
Damon S, Hewitt J, Nieder M, Bennett AB (1988) Sink metabolism in tomato fruit. 11. Phloem unloading and sugar uptake.
Plant Physiol 87: 731-736
Devereaux J, Haeberli P, Smithies O (1984) A comprehensive set of
Dry IB, Robinson SP (1994) Molecular cloning and characterization
of grape berry polyphenol oxidase
Elliott KJ, Butler WO, Dickinson CD, Konno Y, Vedvick TS,
Fitzmaurice L, Mirkov TE (1993) Isolation and characterization
Plant Mo1 Biol 21: 515-524
Eschrich W (1980) Free space invertase, its possible role in phloem
unloading. Ber Dtsch Bot Ges 93: 363-378
Felsenstein J (1989) PHYLIP-phylogeny inference package (Version 3.2)
Frohman A, Dush MK, Martin GR (1988) Rapid production of
full-length cDNAs from rare transcripts: amplification using a
Hawker JS (1969a)Changes in the activities of enzymes concerned
with sugar metabolism during the development of grape berries.
Phytochemistry 8: 9-17
Hawker JS (196913) Insoluble invertase from grapes: an artefact of
extraction. Phytochemistry 8: 337-344
Hedley PE, Machray GC, Davies HV, Burch L, Waugh R (1994)
Higgins DG, Bleasby AJ, Fuchs R (1991) ClustalV: improved
software for multiple sequence alignment
8: 189-191
Ishikawa N, Nakagawa H, Ogura N (1989) Isoforms of invertase
in grape berries. Agric Biol Chem 53: 837-838
Kliewer WM (1965) Changes in concentration of glucose, fructose,
Am J Enol Vitic 16: 101-110
Konno Y, Vedvick T, Fitzmaurice L, Mirkov TE (1993) Purification, characterization, and subcellular location of soluble invertase from tomato fruit
Lang A, During H (1991) Partitioning control by water potential
gradient: evidence for compartmentation breakdown in grape
berries. J Exp Bot 4 0 1069-1078
Leigh RA, ap Rees T, Fuller WA, Banfield J (1979) The location of
Lingle SE, Dunlop JR (1987) Sucrose metabolism in netted muskmelon fruit during development
Marchuk D, Drumm M, Saulino A, Collins SF (1990) Construction of T-vectors, a rapid and general method for direct cloning
of unmodified PCR products
Mercier RW, Gogarten JP (1995) A second cell wall acid invertase
gene in Arabidopsis thaliana
Nakanishi K, Wu W, Yokotsuka K (1991) Purification and some
properties of thermostable invertases from wine
Ohyama A, Ito H, Sato T, Nishimura S, Imai T, Hirai M (1995)
Suppression of acid invertase activity by antisense RNA modifies the sugar composition of tomato fruit
Porntaveewat W, Takayanagi T, Yokotsuka K (1994) Purification
and properties of invertase from Muscat Bailey A grapes. J Ferment Bioeng 7 8 288-292
Rathjen AH, Robinson SP (1992) Aberrant processing of polypheno1 oxidase in a variegated grapevine mutant
Rezaian MA, Krake LR (1987) Nucleic acid extraction and virus
detection in grapevine. J Viro1 Methods 17: 277-285
Roitsch T, Bittner M, Godt DE (1995) Induction of apoplastic
Ruffner HP, Adler S, Rast DM (1990) Soluble and wall associated
forms of invertase in Vitis viniferu
Ruffner HP, Hurlimann M, Skrivan R (1995) Soluble invertase
from grape berries: purification and deglycosylation and antibody specificity
Schowalter DB, Sommer SS (1989) The generation of radiolabelled DNA and RNA probes with polymerase chain reaction.
Ana1 Biochem 177: 90-94
Stommel JR (1992) Enzymic components of sucrose accumulation
Sturm A, Chrispeels MJ (1990) cDNA cloning of carrot extracellular P-fructofuranosidase and its expression in response to
wounding and bacterial infection
Sturm A, Sebkova V, Lorenz K, Hardegger M, Lienhard S, Unger
C (1995) Development and organ-specific expression of the
Swanson CA, Elshishiny EDH (1958) Translocation of sugars in
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