Study of Krebs Cucle Using Mitochondria from Mung Bean Seedlings

Topics: Mitochondrion, Adenosine triphosphate, Cell, Citric acid cycle, Cellular respiration / Pages: 9 (3273 words) / Published: Nov 11th, 2014
Experiment IV: Study of Krebs cycle using Mitochondria from Mung Bean Seedlings

INTRODUCTION
The mitochondria is a very important organelle in the plant cell because it carries out very important cellular reactions in the cell like the Krebs cycle and oxidative phosphorylation which is how the plants produce ATP from the pyruvate produced through glycolysis (Meyer and Millar, 2008). Glycolysis produces a net of 2 ATP for the plant which is not enough for the cell to function while the Krebs cycle and electron transport chain produces a net of 36 ATP which makes the mitochondria the power house of the plant cell (Meyer and Millar, 2008). Mitochondrion is also an important organelle because it contains its own genome and can be transcribed and translated (Meyer and Millar). Mitochondria are isolated to study the Krebs cycle and the proteins that carry out the reaction (Wu and Scheffer, 1960). In addition isolated mitochondria are studied to identify their proteomes and how development affects the proteome (Labbe et al., 2008). Study of isolated mitochondria has great application in the pharmaceutical industry through the discovery that some mitochondrial proteins stimulate apoptosis in the cells (Wang, 2001). This research has aided in finding cure for cancer and Parkinson’s disease among many other diseases (Labbe et al., 2008). Also, many drugs produced contain drug classes that can inhibit the function of mitochondria by preventing or altering a stage in ATP production in the Krebs cycle or electron transport chain or acting as an inhibitor of an enzyme that carries out a certain reaction in citric acid cycle or ETC (Dykens and Will, 2007). These drugs are therefore pretested with isolated mitochondria to reveal any side effects the drug might have (Dykens and Will, 2007).
Most eukaryotic cells are compartmentalized which means that they have specific organelles to carry out specific cellular reactions (Wu and Scheffer, 1960). For example, in plants



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