Purpose: The purpose of this lab experiment is to see if this particular type of fruit which in this case is a strawberry contains DNA. Using materials such as a Ziploc bag to help keep the entire strawberry in one place while it gets smashed and test tube to help better see the DNA and break down of the liquid in the left over strawberry sediments.
Background information: The long thick fibers of DNA store the information for the function of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found can be extracted using common, everyday material. We will use an extraction buffer containing salt, to break up protein chains that bind around the nucleic acids, and dish soap to dissolve the lipid (fat) part of the strawberry cell wall and nuclear membrane. This extraction buffer will help provide us access to the DNA inside the cells.
Pre- Lab Questions:
1. What do you think the DNA will look like?
I think the DNA will look like tiny pink colored little things
2. Where is DNA found?
It is found in the nucleus
Materials: Ziploc bag
5mL DNA extraction buffer \soapy, salty water) Coffee filter
Test tube Inoculating Loop 5mL ethanol
1. Place one good sized strawberry into a Ziploc bag.
2. Zip the bag up and smash or grind up the strawberry by pressing your fingers on the strawberry on the bag for 2 minutes. Careful not to break the bag!!
3. Pour the provided 10mLof extraction buffer (salt and soap solution) into the Ziploc bag.
4. Next mush the strawberry in the Ziploc bag again for another minute.
5. Assemble your filtration apparatus as shown at the top
6. Pour the strawberry slurry from the Ziploc bag and into the filtration apparatus by letting it drip directly into your test tube.
7. Slowly pour cold ethanol into the tube and OBSERVE the results.
8. Dip the loop or glass rod into the tube where the strawberry