Strawberry Dna Extraction Lab
Strawberry DNA Extraction
Donovan Roberts
Mrs. Caudill
Honors Biology
3/7/11
Introduction
The structure of DNA is made of nitrogenous bases, phosphate groups, and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus, bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells can be treated with certain chemicals that cause the membrane to burst. After, the membrane has been busted DNA can be retrieved from the cells. When extracted from a cell DNA appears to be a sticky, clear, algae substance.
Hypothesis
If you add certain chemicals to the beaten cells of a strawberry the sticky DNA can be retrieved from within the cells.
Materials
Zip Lock Bag
Strawberry
Cheesecloth
Funnel
100 ml Beaker
Test Tube
Wooden Coffee Stirrer
DNA Extraction Buffer
Alcohol
Procedure
They are several steps of extracting DNA from strawberry cells. First, you want to ensure that you all have needed materials and are aware of all safety precautions. To being the procedure you start by placing one strawberry in a zip lock baggie making sure all air is sealed out of the bag. To burst the cells of the strawberry you must smash the strawberry with your fist for 2 minutes. After, you have smashed the strawberry you must add 10 ml of extraction buffer to the bag and carefully seal the bag, making sure all air is sealed out. Next, you must smash the strawberry and extraction buffer for a minute. When the both the strawberry and extraction buffer is mixed throughout you must filter the substance through a cheesecloth into the funnel which is connected to the test tube. Fill the test tube 1/8 full and discard of the extra strawberry mixture. After, the test tube is filled with the mixture you must add ice-cold alcohol until the test tube is halfway filled.
Donovan Roberts
Mrs. Caudill
Honors Biology
3/7/11
Introduction
The structure of DNA is made of nitrogenous bases, phosphate groups, and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus, bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells can be treated with certain chemicals that cause the membrane to burst. After, the membrane has been busted DNA can be retrieved from the cells. When extracted from a cell DNA appears to be a sticky, clear, algae substance.
Hypothesis
If you add certain chemicals to the beaten cells of a strawberry the sticky DNA can be retrieved from within the cells.
Materials
Zip Lock Bag
Strawberry
Cheesecloth
Funnel
100 ml Beaker
Test Tube
Wooden Coffee Stirrer
DNA Extraction Buffer
Alcohol
Procedure
They are several steps of extracting DNA from strawberry cells. First, you want to ensure that you all have needed materials and are aware of all safety precautions. To being the procedure you start by placing one strawberry in a zip lock baggie making sure all air is sealed out of the bag. To burst the cells of the strawberry you must smash the strawberry with your fist for 2 minutes. After, you have smashed the strawberry you must add 10 ml of extraction buffer to the bag and carefully seal the bag, making sure all air is sealed out. Next, you must smash the strawberry and extraction buffer for a minute. When the both the strawberry and extraction buffer is mixed throughout you must filter the substance through a cheesecloth into the funnel which is connected to the test tube. Fill the test tube 1/8 full and discard of the extra strawberry mixture. After, the test tube is filled with the mixture you must add ice-cold alcohol until the test tube is halfway filled.