Stirred Tank Bioreactor Reaction Paper

2.1 Bioreactor configuration:
Stirred tank bioreactor is a cylindrical vessel. The semi-circle bottom of the cylindrical vessel was made of stainless steel with an internal diameter of Ti=8 inch, outside diameter 10 inch and height of vessel 24 inch giving it a 3:1 height to diameter ratio for the working volume (Group et al., 2006). The experiments were performed in batch conditions at different temperature, pH and atmospheric pressure. The liquid phase was deionized water (28± 0.5°C). Filtered air was fed to the system through the sparger located 5cm (Ti/2) below the lower impeller. Figure 1 gives the stirred tank bioreactor designed, constructed and utilized in this study.

2.1.1 Jacketed Stainless Steel Vessel:
For laboratory
Gaseous byproducts formed, such as CO2, was removed, and aeration and gas-removal processes take place semi continuously (Williams, 2002). For optimal mixing, there was not only an agitator system but also baffles, which help prevent a whirlpool effect that could impede proper mixing. In the early stages of the process, warm water may be circulated through the baffles to heat up the system; later, cool water may be circulated inside of them to keep the process from overheating. As the bio reaction progresses, the bubbles produced by the air supply are broken up by the agitator as they travel upward. At the top of the tank, exhaust gas is discharged and the product was collected from the bottom. Bacterial growth was followed by reading the optical density (OD) at 600 nm.
2.2.1 pH and Dissolved Oxygen Control:
An acid or alkali is added if the pH needs to be controlled. Dissolved oxygen and pH probes must be inserted into the sterile envelope of the bioreactor. The ability to insert probe for collecting data is a significant consideration. Tank hardware should be designed to accommodate and support probe installation.
Standard electromechanical probes can be used for pH and O2 measurements. They are then integrated into a control system for gas-f low and pump control to ensure that a process value tracks set point. As fluorescent sensor technology advances, patches will be
kefiranofaciens ATCC 8007. The bacterium was activated in Man-Rogosa-Sharpe (MRS) agar medium (Difco, BD Diagnostic Systems, Maryland, USA) consisting of (g L1): peptone casein, 30; meat extract, 10; yeast extract, 6.0; sodium acetate, 5.0; ammonium citrate, 2.0; glucose, 0.2; magnesium sulfate, 0.2; manganese sulfate, 0.05; dipotassium phosphate, 2.0. The pH of the medium was adjusted to 6.5. The master cell bank culture was inoculated into agar plates and incubated for 48 h to produce the working cell culture. Stock cultures were preserved in 50% glycerol (v.v1 )at 78 C (Dailin et al., 2015).
2.2.4 Inoculum preparation:
Inoculum was prepared in a 250 ml Erlenmeyer flask containing 50 ml ATCC 14 broth medium. After sterilization for 15 min at 121˚C, 50 ml YPM medium was inoculated with 250 µl of glycerol culture. The inoculated flasks were incubated on the rotary shaker (Innova 4080, New Brunswick Scientific Co., NJ, USA) at 200 rpm and 30˚C for 24 h. Cells were used thereafter to inoculate either 250 ml Erlenmeyer flasks or stirred tank bioreactor with inoculum concentration of 10% (v/v) (Then et al.,