Starch Digestion by Pancreatic Amylase
Khadijah Lockhart
Biology Lab Report
November 4, 2013
Title: Starch Digestion by Pancreatic Amylase
Statement of the Problem: What happens to enzymes when they are boiled?
If digestion doesn’t occur, which will be present starch or maltose?
If digestion does occur, which will be present starch or maltose?
Hypothesis: I predict that when enzymes are boiled, they will become larger. If digestion does not occur starch will be present, and if it does occur maltose will be present.
Materials:
Test Tube
Boiling Water
Starch Suspension
Pancreatic Amylase Solution
Procedures:
1. Using a wax pencil, number eight clean test tubes 1 through 8 above the level of boiling water bath and mark at 1 and 2cm levels.
2. Fill test tubes 1 through 6 to the 1cm mark with pancreatic amylase solution. Fill tubes 7 and 8 to the 1cm mark with water.
3. Shake the starch suspension each time before dispensing. After tubes 3 through 8 have received the starch suspension; 30 minutes will lapse before testing occurs.
4. Fill tubes 3 and 4 to the 2cm mark with starch suspension and stand for 30 minutes.
5. Place tubes 5 and 6 in boiling water bath for 10 minutes. After boiling, fill to the 2cm mark with starch suspension and let stand for another 20 minutes.
6. Fill tubes 7 and 8 to the 2cm mark with starch suspension. Let tubes stand for 30 minutes.
Results (Data)
*See chart
All contents using Benedict’s test had negative results.
Conclusion:
My hypothesis was correct. The two-test determining starch digestion revealed the presence of maltose or starch in the solutions. This experiment has aided me in learning that everything doesn’t digest the same in the body.
Biology Lab Report
November 4, 2013
Title: Starch Digestion by Pancreatic Amylase
Statement of the Problem: What happens to enzymes when they are boiled?
If digestion doesn’t occur, which will be present starch or maltose?
If digestion does occur, which will be present starch or maltose?
Hypothesis: I predict that when enzymes are boiled, they will become larger. If digestion does not occur starch will be present, and if it does occur maltose will be present.
Materials:
Test Tube
Boiling Water
Starch Suspension
Pancreatic Amylase Solution
Procedures:
1. Using a wax pencil, number eight clean test tubes 1 through 8 above the level of boiling water bath and mark at 1 and 2cm levels.
2. Fill test tubes 1 through 6 to the 1cm mark with pancreatic amylase solution. Fill tubes 7 and 8 to the 1cm mark with water.
3. Shake the starch suspension each time before dispensing. After tubes 3 through 8 have received the starch suspension; 30 minutes will lapse before testing occurs.
4. Fill tubes 3 and 4 to the 2cm mark with starch suspension and stand for 30 minutes.
5. Place tubes 5 and 6 in boiling water bath for 10 minutes. After boiling, fill to the 2cm mark with starch suspension and let stand for another 20 minutes.
6. Fill tubes 7 and 8 to the 2cm mark with starch suspension. Let tubes stand for 30 minutes.
Results (Data)
*See chart
All contents using Benedict’s test had negative results.
Conclusion:
My hypothesis was correct. The two-test determining starch digestion revealed the presence of maltose or starch in the solutions. This experiment has aided me in learning that everything doesn’t digest the same in the body.