Photosynthesis Lab Report:
The Effects of Varying Concentrations of CO2 on the rate of Photosynthesis in Spinach Leaves
Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct.
The purpose of this experiment is to see how different concentrations of carbon dioxide affect the rate of photosynthesis in spinach leaves.
I hypothesize that the greater the concentration of CO2 the faster the plant spinach leaves will photosynthesize because the formula for photosynthesis is: carbon dioxide+ watercell food+ …show more content…
Therefore there will be no variety in leaf size so there is no variable in the amount of CO2 absorbed.
Step 2: Prepare five different solutions of the varying carbon dioxide concentrations. They will range from .2 mL to 1.0 mL. Cup one will be the control with 2%. The carbon dioxide concentration will determine the amount of leaves that will float over a set period of time.
Step 3: in order to wet the hydrophobic surface of the leaf add one drop of liquid soap to each cup, this will allow the solution to be drawn into the leaf and enable the leaves to sink in the fluid.
Step 4: With the plastic syringe draw out the gases of the spongy mesophyll tissue and infiltrate the leaves with sodium bicarbonate solution and then pour the solution into the appropriate cup. By doing this it will cause the leaves to sink.
Step 5: Place the cups under the lamp and record the number of leaves that have floated to the top of the cup each minute. Continue to do this until all the leaves have floated to the top or until there is no activity in the cups anymore. By doing this it will be possible to find the