Spinach Photosynthesis Lab

Topics: Photosynthesis, Color, Light, Wavelength, Spectroscopy, Chemistry / Pages: 7 (1553 words) / Published: Apr 9th, 2017
In this experiment spinach, DPIP, phosphate solutions were placed under various filters and the percent orange light transmittance through the solution was measured to determine the rate of photosynthesis. This experiment was set up to discover how different wavelengths of light affect the rate of photosynthesis in a spinach, DPIP, phosphate solution.
I proposed that the spinach, DPIP, phosphate solution under no filter, and therefore exposed to all light will have a highest percent orange light transmittance and a highest rate of photosynthesis. The solution under the red filter will have the next highest rate of photosynthesis, then the solution under the blue filter, and then the solution under the green filter. This leaves the solution
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The data collected throughout this experiment showed that the spinach, DPIP, phosphate solution under the opaque cup had the lowest percent orange light transmittance and therefore the lowest rate of photosynthesis and the t-Tests supported this. The data collected also showed that the solution under the green filter had the second lowest rate of photosynthesis and the solution under the blue filter had the third lowest rate of photosynthesis. This data supports my hypothesis.
The data also shows that the solution under no filter had the highest rate of photosynthesis and the solution under the red filter had the second highest rate of photosynthesis. Although this matched my hypothesis, the t-Test comparing these two(2) sets of data shows that the solution under the red filter and the solution under no filter have no clear difference and the results could be due to chance.
The results collected during this experiment closely match the results collected during the same experiment completed by Dr. Church’s D Band class. Their results also show that the solutions under the green, blue and opaque filters have the lowest rates of photosynthesis, while the solutions under the red filter and under no filter have the highest rates of

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