Spectroscopy Report

Topics: Spectroscopy, Photon, Excited state Pages: 51 (8131 words) Published: April 13, 2015
University Malaysia Terengganu
Department of Chemical Sciences

KIM3402 Laboratory Reports for Spectrometry

Program: Bachelor of Science (Chemistry)
Lab Group: K1

Prepared for: Dr. Noorashikin Binti MD Saleh

Name of Author:Liew Ken Min
Student ID:UK 31217


CHAPTER 1Theory and Objectives of the experiment 3 1.1 General Properties of Spectroscopic Methods
1.2 UV-Vis Spectroscopy
1.3 Fluorescence Spectroscopy
1.4 Atomic Spectrometric Methods
CHAPTER 2Methodology 14
2.1UV-Vis Spectroscopy
2.2Fluorescence Spectroscopy
2.3Atomic Spectrometric Methods

CHAPTER 3Results and Data Analysis 18
3.1UV-Vis Spectroscopy
3.2Fluorescence Spectroscopy
3.3Atomic Spectrometric Methods

CHAPTER 4Discussions and Problem Solving 33
4.1UV-Vis Spectroscopy
4.2Fluorescence Spectroscopy
4.3Atomic Spectrometric Methods

CHAPTER 5Conclusion 50

CHAPTER 6Bibliography 51

Chapter 1
Theory and Objectives of the experiments

1.1 General Properties of Spectroscopy methods
There are three different major types of spectroscopy techniques covered in our practical, which includes Ultraviolet-Visible Light Spectroscopy, Infrared Spectroscopy and Atomic Absorption Spectroscopy & Atomic Emission Spectroscopy. The use of spectrophotometry in the analysis of chemicals quantitatively and qualitatively is common, since they’re readily available and generally easy to operate, at the same time giving fairly accurate and consistent results. The choice of instrument depends on the wavelength region of choice, which in turn depends on the nature of analyte, either they are coloured or can be changed into coloured derivatives; whether they contains functional group that absorbs light in the UV or Infrared region; or even other absorbing species that depends on the analyte present in the same phase. Generally, spectrometric methods involved the absorption of electromagnetic radiation from an appropriate source by the analyte, and the amount absorbed is related to the concentration of analyte present in the sample solution. Electromagnetic radiation, in strict definition, refers to a form of energy whose behavior is described by the properties of both waves and particles. However, for purposes in spectroscopic analysis, electromagnetic radiation is better defined as a form of radiant energy that propagates as a transverse wave, it vibrates perpendicular to the direction of propagation and this imparts a wave motion. Furthermore, the oscillation of electric and magnetic fields in the propagation is perpendicular to each other. Since electromagnetic radiation is a form of wave motion, unsurprisingly they must possess a certain amount of energy, and it’s carried by the units of radiation which often referred as photons. As a sample absorbs electromagnetic radiation, it undergoes a change in energy, corresponding to the amount of radiation absorbed. Assuming the radiation consists of a beam of energetic particles (photons), the sample acquires the energy of the photons depending on the amount of photons they interacted. The energy of photon is related to frequency of the radiation, by the formula:-

Where E is the energy of the photon, h is Planck’s constant (6.62x10-34 J-s) and v is the velocity of the wave propagation.
There are three different possible ways for a molecule (sample) to absorb radiation, and these three ways of absorption correspond to three distinctive types of transition, which are rotational transition, vibrational transition, and electronic transition. All of these transitions involve raising the molecule to a higher internal energy level, with the increase in energy being equal to the energy of the absorbed radiation. The three types of internal energy are said to be quantized, which means they exist at...

Bibliography: 1. Harvey, D. (2000). Modern analytical chemistry. Boston: McGraw-Hill.
2. Gary D., C. (2004). Analytical Chemistry (Sixth Edition ed.). Boston: McGraw-Hill
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