Spectrophotometric Methods: the Determination of Iron
Spectrophotometric Methods: The Determination of Iron
The goal of this laboratory experiment was the quantitative spectrophotometric determination of Fe (II) by using a calibration plot, which obeyed the Beer-Lambert Law. In order to determine the Fe (II) concentration, a series of solutions of known concentrations were made. The solutions were analyzed by the Ocean Optics spectrophotometer in order to determine their absorbance. The concentration of the unknown Fe (II) was determined by the “eye-ball” method from the Beer’s Law plot of the series of solutions. In this experiment, bipyridine method was used to measure the total iron concentration of an aqueous solution and the reduced iron content in a mineral-enriched cereal. In a similar manner, the concentration of the iron in the cereal was determined from the Beer’s plot by using the “eye-ball” method.
Ocean Optics Spectrophotometric Block Diagram
Source: Is a tungsten-halogen lamp, which emits radiation in the visible region (as well as the near the infrared and long wavelength end of the UV).
Sample Holder: the sample holder that is also called the cuvette is a rectangular tube with two transparent faces. Pyrex glass or plastic cuvettes are transparent to visible light, but fused silica is needed for UV work.
Fiber Optic: it is a flexible cable specifically designed to transmit light to a remote site. These devices are commonly used in telephone transmission lines and for ultra-high speed data transfer in computers. Special miniatures versions are used by physicians to view internal body organs.
Slit: the entrance slit determines the spatial width of the light beam striking and grating.
Grating: disperses the light, which means that the component wavelengths are spread out in space (much like the rainbow effect of a prism).
2048 Miniature Detectors: Each of these detectors has a charged-coupled device. The dispersed bean is passed from the granting projects onto the
The goal of this laboratory experiment was the quantitative spectrophotometric determination of Fe (II) by using a calibration plot, which obeyed the Beer-Lambert Law. In order to determine the Fe (II) concentration, a series of solutions of known concentrations were made. The solutions were analyzed by the Ocean Optics spectrophotometer in order to determine their absorbance. The concentration of the unknown Fe (II) was determined by the “eye-ball” method from the Beer’s Law plot of the series of solutions. In this experiment, bipyridine method was used to measure the total iron concentration of an aqueous solution and the reduced iron content in a mineral-enriched cereal. In a similar manner, the concentration of the iron in the cereal was determined from the Beer’s plot by using the “eye-ball” method.
Ocean Optics Spectrophotometric Block Diagram
Source: Is a tungsten-halogen lamp, which emits radiation in the visible region (as well as the near the infrared and long wavelength end of the UV).
Sample Holder: the sample holder that is also called the cuvette is a rectangular tube with two transparent faces. Pyrex glass or plastic cuvettes are transparent to visible light, but fused silica is needed for UV work.
Fiber Optic: it is a flexible cable specifically designed to transmit light to a remote site. These devices are commonly used in telephone transmission lines and for ultra-high speed data transfer in computers. Special miniatures versions are used by physicians to view internal body organs.
Slit: the entrance slit determines the spatial width of the light beam striking and grating.
Grating: disperses the light, which means that the component wavelengths are spread out in space (much like the rainbow effect of a prism).
2048 Miniature Detectors: Each of these detectors has a charged-coupled device. The dispersed bean is passed from the granting projects onto the