Skeletal Muscle Lab Report
Frog Skeletal Muscle
The aim of this experiment is to explore the basic physiological principles of skeletal muscle using the isolated frog (Rana pipiens or Xenopus laevis) gastrocnemius muscle. Students will dissect a double-pithed frog. Then, they will connect the muscle to the Force Transducer to measure twitch recruitment, effect of stretch, muscle summation, muscle tetanus, and muscle fatigue.
Written by staff of ADInstruments.
Experiment Contents
1. Instructor’s Reference (this document)
This document contains information about modifying the presented experiment depending on the level of your course and proficiency of your students with LabChart software. It also contains information about troubleshooting, analyzing data, equipment alternatives, answers to the report questions, answers to the study questions, …show more content…
Click Start. Follow the settings from step 5.
9. Repeat steps 6-8. Change the Stimulator Frequency to 10 Hz.
10. Repeat steps 6-8 again. Change the Stimulator Frequency to 20 Hz.
11. Repeat steps 6-8 again. Change the Stimulator Frequency to 50 Hz.
12. After you have clicked Start and OK when the Frequency is 50 Hz, Stop Recording the macro. In the Settings File, the macro is called Muscle Tetanus and is assigned the hotkey Command+4. (If you change these settings, you also need to change the Student Protocol.)
Fatigue
As the students will be setting the amplitude on their own, it is important to follow the steps regarding the Stimulator carefully. If you set the Stimulator Amplitude after you start recording the macro, it will not work for the students.
1. Go to the Macro menu and select Start Recording.
2. Go to Stimulator in the Setup menu. Make the following settings in the dialog:
Isolated Stimulator checkbox: unchecked
Stimulator Mode: Pulse
Output: Fixed number of pulses
Number of pulses: 1500
Marker Channel: Channel 2 (Stim Mark.)
Start: When recording starts
Delay: 250 ms
Frequency: 50
The aim of this experiment is to explore the basic physiological principles of skeletal muscle using the isolated frog (Rana pipiens or Xenopus laevis) gastrocnemius muscle. Students will dissect a double-pithed frog. Then, they will connect the muscle to the Force Transducer to measure twitch recruitment, effect of stretch, muscle summation, muscle tetanus, and muscle fatigue.
Written by staff of ADInstruments.
Experiment Contents
1. Instructor’s Reference (this document)
This document contains information about modifying the presented experiment depending on the level of your course and proficiency of your students with LabChart software. It also contains information about troubleshooting, analyzing data, equipment alternatives, answers to the report questions, answers to the study questions, …show more content…
Click Start. Follow the settings from step 5.
9. Repeat steps 6-8. Change the Stimulator Frequency to 10 Hz.
10. Repeat steps 6-8 again. Change the Stimulator Frequency to 20 Hz.
11. Repeat steps 6-8 again. Change the Stimulator Frequency to 50 Hz.
12. After you have clicked Start and OK when the Frequency is 50 Hz, Stop Recording the macro. In the Settings File, the macro is called Muscle Tetanus and is assigned the hotkey Command+4. (If you change these settings, you also need to change the Student Protocol.)
Fatigue
As the students will be setting the amplitude on their own, it is important to follow the steps regarding the Stimulator carefully. If you set the Stimulator Amplitude after you start recording the macro, it will not work for the students.
1. Go to the Macro menu and select Start Recording.
2. Go to Stimulator in the Setup menu. Make the following settings in the dialog:
Isolated Stimulator checkbox: unchecked
Stimulator Mode: Pulse
Output: Fixed number of pulses
Number of pulses: 1500
Marker Channel: Channel 2 (Stim Mark.)
Start: When recording starts
Delay: 250 ms
Frequency: 50