Simple Distillation
Title: Preparation and purification ethyl alcohol
Purpose: The purpose of this experiment is to form a yeast mixture with water, organic compound like sucrose, Ba (OH) 2 for fermentation process, and use simple distillation method to purification from the fermentation mixture.
Abstract and Theory: There are many techniques that are used to purify liquid. The one that is going to use for this lab is simple distillation. It is a process of which separating a mixture based on the tendency of the mixture to vaporize into a boiling liquid. For this particular experiment, a disaccharide carbohydrate sugar like sucrose will mix with yeast a microorganism, water and Ba(OH)2 to form a liquid mixture. The microorganism yeast will convert the sugar …show more content…
In Water | Ethel alcohol | CH3CH2OH | | 46.7 | -114 | 78 | miscible | yeast | | .5 pack | | | | | Sucrose | C12H22O11 | 51.1g | 342.40 | 186 | | 2000g/L | Barium hydroxide | Ba(OH)2 | Small amount | 171.34 | 78 | 780 | 1.67g/100ml | Disodium hydrogen phosphate | Na2HPO4 | .35 g | 141 | 250 | | 11.8g/100ml | Celite | | 10 g | | | | | …show more content…
Added 1/2 pack of yeast and a few ml of water in a 100 ml beaker and mixed to form paste. 2. Added water until the overall volume reach about 50 ml so the yeast had adequate nutrient and mixed the solution. 3. Added .35g of Na2 PO4 in the yeast mixture as a nutrient source. 4. Transferred the mixture into a 500 ml round bottom flask. 5. Added 51.5 g of sucrose in 200 ml of water and mixed the solution. 6. Transferred the solution to the yeast mixture, swirl the flask, and mixed all the compounds. 7. Added a few ml of Ba (OH) 2 in a test tube for the fermentation reaction. 8. Closed the flask with a rubber stopper with a glass tube that reached the Ba (OH) 2 solution for the fermentation process.
Week two: 9. Added 10 g of Celite to the 500 ml flask to help prevented clogged in the yeast mixture and mixed it. 10. Waited 5 minutes for the solid descend on the bottom of the flask. 11. Transferred the mixture into a large Buchner funnel with the filter flask connected to the vacuum filtration system for filtration. 12. Collected and transferred the filter ferment liquid in a 500 ml round bottom flask and Added a few boiling chips to aid the boiling
Purpose: The purpose of this experiment is to form a yeast mixture with water, organic compound like sucrose, Ba (OH) 2 for fermentation process, and use simple distillation method to purification from the fermentation mixture.
Abstract and Theory: There are many techniques that are used to purify liquid. The one that is going to use for this lab is simple distillation. It is a process of which separating a mixture based on the tendency of the mixture to vaporize into a boiling liquid. For this particular experiment, a disaccharide carbohydrate sugar like sucrose will mix with yeast a microorganism, water and Ba(OH)2 to form a liquid mixture. The microorganism yeast will convert the sugar …show more content…
In Water | Ethel alcohol | CH3CH2OH | | 46.7 | -114 | 78 | miscible | yeast | | .5 pack | | | | | Sucrose | C12H22O11 | 51.1g | 342.40 | 186 | | 2000g/L | Barium hydroxide | Ba(OH)2 | Small amount | 171.34 | 78 | 780 | 1.67g/100ml | Disodium hydrogen phosphate | Na2HPO4 | .35 g | 141 | 250 | | 11.8g/100ml | Celite | | 10 g | | | | | …show more content…
Added 1/2 pack of yeast and a few ml of water in a 100 ml beaker and mixed to form paste. 2. Added water until the overall volume reach about 50 ml so the yeast had adequate nutrient and mixed the solution. 3. Added .35g of Na2 PO4 in the yeast mixture as a nutrient source. 4. Transferred the mixture into a 500 ml round bottom flask. 5. Added 51.5 g of sucrose in 200 ml of water and mixed the solution. 6. Transferred the solution to the yeast mixture, swirl the flask, and mixed all the compounds. 7. Added a few ml of Ba (OH) 2 in a test tube for the fermentation reaction. 8. Closed the flask with a rubber stopper with a glass tube that reached the Ba (OH) 2 solution for the fermentation process.
Week two: 9. Added 10 g of Celite to the 500 ml flask to help prevented clogged in the yeast mixture and mixed it. 10. Waited 5 minutes for the solid descend on the bottom of the flask. 11. Transferred the mixture into a large Buchner funnel with the filter flask connected to the vacuum filtration system for filtration. 12. Collected and transferred the filter ferment liquid in a 500 ml round bottom flask and Added a few boiling chips to aid the boiling