Silver Nanoparticle Lab Report
1- Synthesis of silver nanoparticles by Bacillus sp.:
The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp., on the basis of morphological and biochemical characteristics.
Gram’s staining:
Gram positive and rod shape.
They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution.
Visual identification:
When bacterial biomass mixed with silver nitrate solution (pH 7.5) and incubated at 37° C for 24hours. It shows no change in color. The appearance of milkfish white color is a clear indication of the formation of silver nanoparticle in the reaction mixture …show more content…
platensis with silver nitrate aqueous solution for 2 days. However patterns of nanoparticles are also visualized. It should be noted that the substance of crystalline silver was not high in tested samples. It was no more than 1 %, suggesting that silver nanoparticles were produced, extracellularly. The grains of silver nano-crystals had small size which caused the broadening of Peaks.
3- Synthesis of silver nanoparticles by actinobacteria Streptomyces spp. 211A:
UV−Vis spectrum recorded from the Streptomyces spp. 211A – silver nitrate aqueous solution at 9 days is presented. Fig. also shows the strong absorption peak characteristic to silver nanoparticles. UV–Vis spectra of biomass of Streptomyces spp. 211A treated with 10–3 M AgNO3 aqueous solution for 9 days.
The observation of peak at 425 nm in the aqueous silver nitrate – Sp. platensis and silver nitrate – Streptomyces spp. 211A reaction medium indicates the extracellular synthesis of silver nanoparticles.
Diffractogram of Sp. platensis biomass after exposure to 10–3 M AgNO3 aqueous solution for 2 days.
4- Extracellular synthesis of silver nanoparticles by the Bacillus strain
The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp., on the basis of morphological and biochemical characteristics.
Gram’s staining:
Gram positive and rod shape.
They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution.
Visual identification:
When bacterial biomass mixed with silver nitrate solution (pH 7.5) and incubated at 37° C for 24hours. It shows no change in color. The appearance of milkfish white color is a clear indication of the formation of silver nanoparticle in the reaction mixture …show more content…
platensis with silver nitrate aqueous solution for 2 days. However patterns of nanoparticles are also visualized. It should be noted that the substance of crystalline silver was not high in tested samples. It was no more than 1 %, suggesting that silver nanoparticles were produced, extracellularly. The grains of silver nano-crystals had small size which caused the broadening of Peaks.
3- Synthesis of silver nanoparticles by actinobacteria Streptomyces spp. 211A:
UV−Vis spectrum recorded from the Streptomyces spp. 211A – silver nitrate aqueous solution at 9 days is presented. Fig. also shows the strong absorption peak characteristic to silver nanoparticles. UV–Vis spectra of biomass of Streptomyces spp. 211A treated with 10–3 M AgNO3 aqueous solution for 9 days.
The observation of peak at 425 nm in the aqueous silver nitrate – Sp. platensis and silver nitrate – Streptomyces spp. 211A reaction medium indicates the extracellular synthesis of silver nanoparticles.
Diffractogram of Sp. platensis biomass after exposure to 10–3 M AgNO3 aqueous solution for 2 days.
4- Extracellular synthesis of silver nanoparticles by the Bacillus strain